UNG
DNA Glycosilase Uracil

Synonyms:
Uracil DNA Glycosilase, Uracil DNA Glycosylase,UNG.

Description:
E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).

Source:
Escherichia Coli strain that carries the UNG gene from E.coli

Physical Appearance:
Sterile Filtered colorless solution.

Formulation:
10mM Tris-Hcl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.

Stability:
Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.

Applications:
Glycosilase mediated single nucleotide polymorphism detection (GMPD).
Site-directed mutagenesis.
As a probe for protein-DNA interaction sudies.
Rapid and efficient cloning of PCR products.
Elimination carry-over contamination in PCR.

Unit Definition:
1 Unit is defined as the amount of UDG enzyme that catalyzes release of 60pmol of Uracil per minute from double-stranded, uracil-containing DNA in a total reaction volume of 50µl in 30 minutes at 37°C in 1X Uracil DNA Glycosilase Reaction Buffer with 1 Unit of Uracil DNA Glycosylase and 0.2µg [3H]-Uracil DNA (10^4- 10⁵ cpm/µg)

10X UNG Reaction Buffer:
200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA.

Reaction Conditions:
1X UNG Reaction Buffer, incubate at 37°C.
UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.