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PTPRN HumanDescription:
Protein Tyrosine Phosphatase Receptor Type N Human Recombinant
Receptor-type tyrosine-protein phosphatase-like N, R-PTP-N, Islet cell antigen 512, ICA 512, Islet cell autoantigen 3, PTP IA-2, PTPRN, ICA3, ICA512.
Product # :
ENZ-1162Price :
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UFC1 HumanDescription:
Ubiquitin Fold Modifier Conjugating Enzyme 1 Human Recombinant
Ubiquitin-fold modifier-conjugating enzyme 1, Ufm1-conjugating enzyme 1, UFC1, CGI-126, HSPC155.
Product # :
ENZ-138Price :
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UCHL5 HumanDescription:
Ubiquitin Carboxyl-Terminal Esterase L5 Human Recombinant
Ubiquitin Carboxyl-terminal Hydrolase L5, UCH37, CGI-70, UCH-L5, INO80R, Ubiquitin thioesterase L5, INO80 complex subunit R, EC 3.4.19.12, Ubiquitin Carboxyl-terminal Esterase L5.
Product # :
ENZ-097Price :
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UCHL3 MouseDescription:
Ubiquitin Carboxyl-Terminal Esterase L3 Mouse Recombinant
Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, UCHL3, Ubiquitin Carboxyl-Terminal Esterase L3, Ubiquitin thioesterase L3, Uchl3, Ubiquitin carboxyl-terminal esterase L3.
Product # :
ENZ-978Price :
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UCHL3 HumanDescription:
Ubiquitin Carboxyl-Terminal Esterase L3 Human Recombinant
Ubiquitin Carboxyl-Terminal Esterase L3 (ubiquitin thiolesterase), UCH-L3, Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3, EC 3.4.19.12.
Product # :
ENZ-057Price :
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Name :
UBE2Z HumanDescription:
Ubiquitin Conjugating Enzyme E2Z Human Recombinant
HOYS7, USE1, Ubiquitin-conjugating enzyme E2 Z, E2 ubiquitin-conjugating enzyme Z, Uba6-specific E2 conjugating enzyme 1, Ubiquitin carrier protein Z, Ubiquitin-protein ligase Z, UBE2Z.
Product # :
ENZ-804Price :
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UBE2W HumanDescription:
Ubiquitin Conjugating Enzyme E2W Human Recombinant
Probable ubiquitin-conjugating enzyme E2 W, Ubiquitin carrier protein W, Ubiquitin-protein ligase W, UBE2W, hUBC-16, FLJ11011.
Product # :
ENZ-117Price :
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UBE2V2 HumanDescription:
Ubiquitin-Conjugating Enzyme E2 Variant 2 Human Recombinant
DDVit-1, DDVIT1, EDAF-1, EDPF-1, EDPF1, MMS2, UEV-2, UEV2, Ubiquitin-conjugating enzyme E2 variant 2, Enterocyte differentiation-associated factor 1, Enterocyte differentiation-promoting factor 1, Vitamin D3-inducible protein, UBE2V2.
Product # :
ENZ-550Price :
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UBE2T HumanDescription:
Ubiquitin-Conjugating Enzyme E2T Human Recombinant
EC 6.3.2.19, HSPC150, PIG50, Ubiquitin-conjugating enzyme E2 T, Ubiquitin-protein ligase T, Ubiquitin carrier protein T, Cell proliferation-inducing gene 50 protein, UBE2T.
Product # :
ENZ-506Price :
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UBE2S HumanDescription:
Ubiquitin Conjugating Enzyme E2S Human Recombinant
Ubiquitin-conjugating enzyme E2 S, Ubiquitin-protein ligase S, Ubiquitin carrier protein S, Ubiquitin-conjugating enzyme E2-24 kDa, E2-EPF5, E2-EPF, UBE2S, E2EPF, EPF5.
Product # :
ENZ-444Price :
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UBE2R2 HumanDescription:
Ubiquitin Conjugating Enzyme E2R 2 Human Recombinant
CDC34B, E2-CDC34B, UBC3B, Ubiquitin carrier protein R2, Ubiquitin-conjugating enzyme E2-CDC34B, Ubiquitin-protein ligase R2, Ubiquitin-conjugating enzyme E2 R2, EC 6.3.2.19.
Product # :
ENZ-511Price :
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UBE2Q2 HumanDescription:
Ubiquitin Conjugating Enzyme E2Q2 Human Recombinant
Ubiquitin-conjugating enzyme E2 Q2, UBE2Q2, E2 ubiquitin-conjugating enzyme Q2, Ubiquitin carrier protein Q2, Ubiquitin-protein ligase Q2, Ubiquitin Conjugating Enzyme E2Q2, Ubiquitin-conjugating enzyme E2 Q2 isoform1.
Product # :
ENZ-885Price :
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UBE2N HumanDescription:
Ubiquitin Conjugating Enzyme E2N Human Recombinant
Ubiquitin-conjugating enzyme E2 N, Bendless-like ubiquitin-conjugating enzyme, Ubc13, Ubiquitin carrier protein N, Ubiquitin-protein ligase N, UBE2N, BLU, MGC8489, UbcH-ben, MGC131857.
Product # :
ENZ-104Price :
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UBE2M HumanDescription:
Ubiquitin Conjugating Enzyme E2M Human Recombinant
NEDD8-conjugating enzyme Ubc12, Ubiquitin-conjugating enzyme E2 M, NEDD8 protein ligase, NEDD8 carrier protein, UBC12, hUbc12, UBC-RS2.
Product # :
ENZ-345Price :
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UBE2L6 HumanDescription:
Ubiquitin Conjugating Enzyme E2L 6 Human Recombinant
RIG-B, UBCH8, MGC40331, UBE2L6, Ubiquitin/ISG15-conjugating enzyme E2 L6, Ubiquitin-protein ligase L6, Ubiquitin carrier protein L6, Retinoic acid-induced gene B protein.
Product # :
ENZ-347Price :
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UBE2L3 Human HisDescription:
Ubiquitin-Conjugating Enzyme E2L 3 Human Recombinant, His Tag
Ubiquitin-conjugating enzyme E2 L3, EC 6.3.2.19, Ubiquitin-protein ligase L3,Ubiquitin carrier protein L3, UbcH7, E2-F1, L-UBC, UbcM4.
Product # :
ENZ-344Price :
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UBE2L3 HumanDescription:
Ubiquitin-Conjugating Enzyme E2L 3 Human Recombinant
Ubiquitin-conjugating enzyme E2 L3, EC 6.3.2.19, Ubiquitin-protein ligase L3,Ubiquitin carrier protein L3, UbcH7, E2-F1, L-UBC, UbcM4.
Product # :
ENZ-342Price :
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UBE2K HumanDescription:
Ubiquitin-Conjugating Enzyme E2K Human Recombinant
HIP2, Huntingtin Interacting protein 2, HYPG, Ubiquitin-conjugating enzyme E2-25K kDa, Ubiquitin-protein ligase, Ubiquitin carrier protein, LIG, HIP-2, E2(25K), DKFZp686J24237, OTTHUMP00000218440, EC 6.3.2.19.
Product # :
ENZ-339Price :
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UBE2J2 HumanDescription:
Ubiquitin-Conjugating Enzyme E2, J2 Human Recombinant
Ubiquitin-conjugating enzyme E2 J2, ubiquitin-conjugating enzyme E2 J2 (UBC6 homolog east), Non-canonical ubiquitin-conjugating enzyme 2, ubiquitin conjugating enzyme 6, PRO2121, yeast UBC6 homolog, Ubc6p, NCUBE-2, EC 6.3.2.19.
Product # :
ENZ-626Price :
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Name :
UBE2I Human HisDescription:
Ubiquitin-Conjugating Enzyme E2I Human Recombinant, His Tag
SUMO-conjugating enzyme UBC9, EC 6.3.2.-, SUMO-protein ligase, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, UBC9, C358B7.1.
Product # :
ENZ-274Price :
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Name :
UBE2I HumanDescription:
Ubiquitin-Conjugating Enzyme E2I Human Recombinant
SUMO-conjugating enzyme UBC9, EC 6.3.2.-, SUMO-protein ligase, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, UBC9, C358B7.1.
Product # :
ENZ-341Price :
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UBE2H HumanDescription:
Ubiquitin-Conjugating Enzyme E2H Human Recombinant
Ubiquitin-conjugating enzyme E2 H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, UBE2H, GID3, UBC8, UBCH.
Product # :
ENZ-603Price :
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UBE2G2 HumanDescription:
Ubiquitin-Conjugating Enzyme E2G2 Human Recombinant
Ubiquitin-conjugating enzyme E2 G2, E2 ubiquitin-conjugating enzyme G2, Ubiquitin carrier protein G2, Ubiquitin-protein ligase G2, UBE2G2, Ubiquitin-Conjugating Enzyme E2G2, Ubiquitin-conjugating enzyme E2 G2 isoform 1, UBC7.
Product # :
ENZ-884Price :
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UBE2G HumanDescription:
Ubiquitin-Conjugating Enzyme E2G Human Recombinant
E217K, UBC7, UBE2G, Ubiquitin-conjugating enzyme E2 G1, E2 ubiquitin-conjugating enzyme G1, E217K, UBC7, Ubiquitin carrier protein G1, Ubiquitin-protein ligase G1.
Product # :
ENZ-838Price :
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UBE2F HumanDescription:
Ubiquitin-Conjugating Enzyme E2F Human Recombinant
Ubiquitin-conjugating enzyme E2F (putative), NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, NEDD8-conjugating enzyme UBE2F, NCE2, EC 6.3.2.-.
Product # :
ENZ-625Price :
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UBE2E3 HumanDescription:
Ubiquitin Conjugating Enzyme E2E3 Human Recombinant
UBCH9, UbcM2, UBE2E3, Ubiquitin-Conjugating Enzyme E2E 3, UBCH9, Ubiquitin-Conjugating Enzyme E2E 3 (Homologous To Yeast UBC4/5), Ubiquitin-Conjugating Enzyme E2E 3 (UBC4/5 Homolog, Yeast), Ubiquitin-Conjugating Enzyme E2-23 KDa, Ubiquitin Carrier Protein E3, Ubiquitin-Protein Ligase E3, EC 6.3.2.19, Ubiquitin-Conjugating Enzyme E2 E3, UBCE4.
Product # :
ENZ-906Price :
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UBE2E1 HumanDescription:
Ubiquitin Conjugating Enzyme E2E1 Human Recombinant
Ubiquitin carrier protein E1, Ubiquitin-conjugating enzyme E2E 1 (homologous to yeast UBC4/5), Ubiquitin-protein ligase E1, UBCH6, EC 6.3.2.19.
Product # :
ENZ-647Price :
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UBE2D4 HumanDescription:
Ubiquitin Conjugating Enzyme E2D4 Human Recombinant
Ubiquitin-conjugating enzyme E2D 4 (putative), UBCH5D, HBUCE1, Ubiquitin carrier protein D4, Ubiquitin-protein ligase D4, EC 6.3.2.19.
Product # :
ENZ-179Price :
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Name :
UBE2D3 HumanDescription:
Ubiquitin Conjugating Enzyme E2D3 Human Recombinant
Ubiquitin-conjugating enzyme E2 D3, EC 6.3.2.19, Ubiquitin-protein ligase D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating enzyme E2-17 kDa 3, E2(17)KB 3, UBC4/5, UBCH5C, MGC5416, MGC43926, UBE2D3.
Product # :
ENZ-343Price :
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Name :
UBE2D2 Human, HisDescription:
Ubiquitin Conjugating Enzyme E2D2 Human Recombinant, His Tag
ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5), UBC4, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, UBCH5B, EC 6.3.2.19, E2(17)KB2, PUBC1, UBC4/5, UBC5B, UBCH4.
Product # :
ENZ-154Price :
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Name :
UBE2D2 HumanDescription:
Ubiquitin Conjugating Enzyme E2D2 Human Recombinant
Ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5), UBC4, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, UBCH5B, EC 6.3.2.19, E2(17)KB2, PUBC1, UBC4/5, UBC5B, UBCH4.
Product # :
ENZ-1036Price :
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UBE2D1 HumanDescription:
Ubiquitin Conjugating Enzyme E2D1 Human Recombinant
Ubiquitin-conjugating enzyme E2 D1, Stimulator of Fe transport, SFT, UBC4/5 homolog, UbcH5, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase D1, UBE2D1, SFT, UBC5A, UBCH5A, E2(17)KB1.
Product # :
ENZ-116Price :
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UBE2C HumanDescription:
Ubiquitin Conjugating enzyme E2C Human Recombinant
Ubiquitin-conjugating enzyme E2 C, EC 6.3.2.19, Ubiquitin-protein ligase C, Ubiquitin carrier protein C, Ubc10, UBCH10, dJ447F3.2.
Product # :
ENZ-346Price :
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UBE2B HumanDescription:
Ubiquitin Conjugating Enzyme E2B Human Recombinant
Ubiquitin-conjugating enzyme E2 B, EC 6.3.2.19, Ubiquitin-protein ligase B, Ubiquitin carrier protein B, HR6B, hHR6B, E2-17 kDa UBC2, HHR6B, RAD6B, E2-17kDa, UBE2B.
Product # :
ENZ-340Price :
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UBE2A HumanDescription:
Ubiquitin Conjugating Enzyme E2A Human Recombinant
HHR6A, HR6A, RAD6A, UBC2, UBE2A, EC=6.3.2.19, Ubiquitin-conjugating enzyme E2 A, Ubiquitin-protein ligase A, Ubiquitin carrier protein A, RAD6 homolog A.
Product # :
ENZ-514Price :
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UBA5 HumanDescription:
Ubiquitin-Like Modifier Activating Enzyme 5 Human Recombinant
Ubiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme 5, ThiFP1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1, UBA5, UBE1DC1.
Product # :
ENZ-602Price :
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UBA3 HumanDescription:
Ubiquitin-Like Modifier Activating Enzyme 3 Human Recombinant
NEDD8-activating enzyme E1 catalytic subunit, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3, UBA3, UBE1C, hUBA3.
Product # :
ENZ-576Price :
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UBA2 HumanDescription:
Ubiquitin-Like Modifier Activating Enzyme 2 Human Recombinant
SAE2, UBA-2, SAE-2, SUMO-1 Activating Enzyme Subunit 2.
Product # :
ENZ-959Price :
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NEDD8 HumanDescription:
Neural Precursor Cell Expressed Developmentally Down-Regulated 8 Human Recombinant
Nedd-8, FLJ43224, MGC104393, MGC125896, MGC125897, NEDD8, Ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8.
Product # :
ENZ-396Price :
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SAE1/SAE2 HumanDescription:
SAE1/SAE2 Human Recombinant
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ENZ-1161Price :
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SAE1 HumanDescription:
SUMO1 Activating Enzyme Subunit 1 Human Recombinant
AOS1, HSPC140, SUA1, UBLE1A, SAE1, SUMO1 Activating Enzyme Subunit 1, FLJ3091.
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ENZ-534Price :
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About Ubiquitin Conjugating Enzyme:
Ubiquitin-conjugating enzymes catalyze the second step of the ubiquitination reaction that enables the degradation of proteins via the proteasome.
Ubiquitin Conjugating Enzyme Mechanism
The purpose of the ubiquitin-conjugating enzyme is to help the cell recycle short peptide fragments.
The first step is to attach ubiquitin - a relatively short protein made of 76 amino acids - to the lysine on the target protein using a covalent reaction. Once the initial ubiquitin molecule is in place, and the protein has been tagged, it is then possible for the polyubiquitin chain to form. The proteasome’s 19S regulatory particle recognizes the chain. The proteasome recognizes the chain, triggering the unfolding of the target protein via ATP and allowing passage to the proteasome’s 20S core particle. Proteases then degrade the target into short peptide chains that the cell can use.
Ubiquitin Conjugating Enzyme Function
There are three classes of ubiquitin-related enzymes: E1, E2, and E3. All of these classes have different properties and operate in distinct ways in the body.
E1 is the least physiologically-regulated of all of the enzymes in this class. It activates ubiquitin using the ATP-dependent mechanism described in the previous section. The ATP-activated ubiquitins then bind to the protein substrate and degrade them using the ubiquitin-proteasome pathway. The process is not dependent on the substrate.
E2 ubiquitin-conjugating enzymes are believed to be more selective in their choice of target. Researchers initially thought that the purpose of E2 enzymes was simply to transfer ubiquitin to E3s. However, they may play a more active role. Current evidence suggests that E2s may be able to directly conjugate ubiquitin into substrates.
Unfortunately, a definitive answer to this question is likely a long way from being answers. E2s are structurally diverse and found in different forms across a range of species. In yeast, for instance, 13 genes encode for E2s. In mammals, there appears to be in the order of 25 to 30.
E3s are widely believed to be even more diverse than E2s. In combination, there is evidence that they may have a high degree of site-specificity, allowing them to work on a range of substrates without damaging surrounding tissue.
E3s are so diverse that they may use catalytic mechanisms not described above. Other possible chemical mechanisms could break proteins down into their peptide constituents.
Ubiquitin-conjugating enzymes should not be confused with ubiquitin-activating enzymes. Ubiquitin-activating enzymes are those described above that are not substrate-specific. Their role is to active the ubiquitin by covalently attaching the molecule to the protein site cysteine residue. Once that has occurred, it is then time for the E2 and E3 conjugating enzymes to take over the process. After conjugation, the E2 molecule binds to several of the ubiquitin ligases or the E3 via the unique binding region.
The job of the E3 ubiquitin-conjugating enzyme is to bind to the target protein substrate and then transfer the ubiquitin from the cysteine residue to a new lysine residue on the surface of the target protein.