Ubc9 Human His
Ubiquitin Conjugating Enzyme 9 Human Recombinant, His

Synonyms:
SUMO-conjugating enzyme UBC9, EC 6.3.2.-, SUMO-protein ligase, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, UBC9, C358B7.1.

Introduction:
Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IκBα, and PML without the requirement of an E3 ligase.

Description:
Ubiquitin Conjugating Enzyme 9 Human Recombinant produced in E.coli is a 19.5 kDa protein containing 171 amino acids.
The Ubc9 protein contains 6xHis tag and is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered white lyophilized powder.

Formulation:
Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5.

Solubility:
It is recommended to reconstitute the lyophilized Ubc9 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Ubc9 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Ubc9 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE.

Amino acid sequence:
MHHHHHHAMGTLNMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMN WECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPS
GTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQN
RVEYEKRVRAQAKKFAPS.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.