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Protein Catalog >> Enzymes >> UBE2T Human
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Description
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Ubiquitin-Conjugating Enzyme E2T Human Recombinant
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Catalogue Number ENZ-506
Synonyms EC 6.3.2.19, HSPC150, PIG50, Ubiquitin-conjugating enzyme E2 T, Ubiquitin-protein ligase T, Ubiquitin carrier protein T, Cell proliferation-inducing gene 50 protein, UBE2T.
Introduction UBE2T is part of the E2 ubiquitin-conjugating enzyme family that participates in the protein degradation pathway. UBE2T catalyzes the ATP-dependent attachment of ubiquitin to target proteins, thus tagging them for subsequent destruction by the proteasome. UBE2T is an important factor of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway.
Description UBE2T Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 205 amino acids (1-197 a.a.) and having a molecular mass of 23.6 kDa. The UBE2T is fused to an 8 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered clear colorless solution.
Formulation The UBE2T solution contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Amino acid sequence MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDVLEH HHHHH.
Purity Greater than 95% as determined by SDS-PAGE.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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