Thymosin β4
Thymosin β4

Synonyms:
Thymosin beta-4, T beta 4, Fx , TB4X, PTMB4, TMSB4.

Introduction:
Thymosin is a hormone secreted from the thymus. Its primary function is to stimulate the production of T cells, which are an important part of the immune system.
Thymosin also assists in the development of B cellsto plasma cellsto produce antibodies.
The predominant form of thymosin, thymosin β4, is a member of a highly conserved family of actinmonomer-sequestering proteins. β-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmicpool of free G-actinmonomersrequired for rapid filament elongation and allowing for the flux of monomers between the thymosin-bound pool and F-actin.

Description:
Thymosin b₄ is a 43 amino acid peptide which is regarded as the main intracellular G-actin sequestering peptide.It has a molecular weight of 4963.49 Da, and its molecular formula is: C₂₁₂H₃₅₀N₅₆O₇₈S₁ Extracellular Thymosin b₄ may contribute to physiological processes such as angiogenesis, wound healing, and regulation of inflammation.
Thymosin b₄ has an a.a. sequence of Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH.

Formulation:
The protein (1 mg/ml) was lyophilized with no additives.

Solubility:
It is recommended to reconstitute the lyophilized Thymosin beta-4 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Thymosin b₄ although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution T beta 4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.