Threonyl tRNA Synthetase Human

Description

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Threonyl-tRNA Synthetase Human Recombinant

	5µg($50)
	20µg($130)
	1mg($3900)

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Catalogue Number

ENZ-304

Synonyms

Threonyl-tRNA synthetase cytoplasmic, EC 6.1.1.3, Threonine-tRNA ligase, ThrRS, MGC9344, PL-7, TARS.

Introduction

Threonyl-tRNA Synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 83 kDa protein is an autoantigen recognized by PL-7 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that PL-7 antibodies (similar to Jo-1 antibodies) indicate an increased risk for lung involvement, but this needs to be confirmed for a larger number of cases.

Description

Threonyl-tRNA Synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 83,312 Dalton.
PL-7 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Source

Sf9 insect cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

PL-7 is supplied in 20mM HEPES buffer pH-8, 200mM sodium chloride, and 20% glycerol.

Immunological functions

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).

Applications

Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.

Coating concentration

0.3-0.8 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.