Thioredoxin Reductase Yeast

Description

Our Price

Quantity

Shipping

Thioredoxin Reductase (NADPH) Yeast Recombinant

	5µg($50)
	20µg($130)
	1mg($3200)

Catalogue Number

ENZ-278

Synonyms

Thioredoxin Reductase (NADPH), NTR, TrxR.

Introduction

Thioredoxin reductase (TrxR/NTR), an enzyme belonging to the flavoprotein family of pyridine nucleotide-disulfide oxidoreductases. Thioredoxin reductase (TrxR), a component of the thioredoxin system, including thioredoxin (Trx) and NADPH, catalyzes the transfer of electrons from NADPH to Trx, acts as a reductant of disulfide-containing proteins and participates in the defense system against oxidative stresses.

Description

Thioredoxin Reductase (NADPH) Yeast Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 36 kDa.
Thioredoxin Reductase is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Each mg of protein contains 20mM phosphate buffer pH 7.4 and 0.15M sodium chloride.

Solubility

It is recommended to reconstitute the lyophilized NTR in sterile 18MΩ-cm H2O.

Stability

NTR although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
Please prevent freeze thaw cycles.

Purity

Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity

The specific activity was found to be 5 IU/mg.

Unit Definition

One unit equals the change in absorbance at 412 nm per minute at 25°C using 0.2mM NADPH containing 5mM DTNB (pH 7.0).

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.