- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-837
Synonyms
Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.
Introduction
Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
Description
TGFB1 Human Recombinant produced in CHO cells is a homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25kDa.
Source
Chinese Hamster Ovary cells.
Physical Appearance
Sterile filtered colorless solution.
Formulation
The TGF b 1 Human 0.22 µm filtered solution (0.24mg/ml) contains 20mM TRIS HCl buffer pH 7.2.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Amino acid sequence
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Biological Activity
The ED50 for this effect is typically 20-60ng/ml before activation and 0.1-1ng/ml after activation as measured by TGF-b1’s ability to inhibit the mouse IL-4-dependent proliferation of HT-2 cells.
Activation: Activated in vitro at pH below 3.5 and over 12.5.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.