TGF b 1 Human Recombinant

Description

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Quantity

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Transforming Growth Factor-Beta 1 Human Recombinant

	1µg($50)
	5µg($130)
	100µg($1500)

Catalogue Number

CYT-716

Synonyms

Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.

Introduction

Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.

Description

TGFB1 Human Recombinant produced in Human 293 cells is a homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25kDa. The TGFB1 is purified by proprietary chromatographic techniques.

Source

Human 293 cells.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a concentrated (1mg/ml) solution containing 10mM HCl & 50ug BSA.

Solubility

It is recommended to reconstitute the lyophilized TGFB1 in sterile 5mM HCl & 50ug/ml BSA at a concentration of 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB1 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by SDS-PAGE.

Amino acid sequence

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.

Biological Activity

The ED50, as measured by the dose-dependent inhibition of IL-4-induced proliferation of mouse HT-2 cells, was found to be 0.126 ng/ml, corresponding to a specific activity of 7,936,508 Units/mg.

References

Title:Imatinib attenuates skeletal muscle dystrophy in mdx mice .
Publication:Published online before print March 16, 2009, doi: 10.1096/fj.09-129833 August 2009 The FASEB Journal vol. 23 no. 8 2539-2548 .
Link:http://www.fasebj.org/content/23/8/2539.full

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.