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Catalogue Number
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CYT-473
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Synonyms
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Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB.
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Introduction
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Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
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Description
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The TGF-b 1 (177-391 a.a.) is purified by standard chromatographic techniques and shows a 51 kDa band on SDS-PAGE (including GST tag).
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered solution at a concentration of 0.1 mg/ml.
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Formulation
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The protein solution contains 50mM Tris-Acetate, pH-7.5, 1mM EDTA and 20% Glycerol.
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Stability
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TGF-beta 1 although stable at 4°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
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Purity
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Greater than 80.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
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Amino acid sequence
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The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn.
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Usage
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Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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