TDGF1 Human | ProSpec

Description

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Quantity

Shipping

Teratocarcinoma-Derived Growth Factor 1 Human Recombinant

	2µg($50)
	10µg($130)
	1mg($5200)

Catalogue Number

CYT-712

Synonyms

CRIPTO1, CRIPTO-1, CRGF, Teratocarcinoma-Derived Growth Factor 1, TDGF1, CR.

Introduction

TDGF1 is an extracellular, membrane-bound signaling protein that is involved in embryonic development and tumor growth. Mutations in TDGF1 are associated with forebrain defects TDGF1 takes part in the determination of the epiblastic cells that later provide growth to the mesoderm.

Description

TDGF1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 139 amino acids with a molecular weight of 17.8 kDa though on SDS-PAGE migrates at about 25kDa due to the glycosilation. The TDGF1 is purified by proprietary chromatographic techniques.

Source

HEK293-F Cells.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The TDGF1 protein was lyophilized from a 0.2µm filtered solution containing no additives or preservatives.

Solubility

It is recommended to reconstitute the lyophilized TDGF1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TDGF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TDGF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Amino acid sequence

LGHQEFARPS RGYLAFRDDS IWPQEEPAIR PRSSQRVPPM GIQHSKELNR TCCLNGGTCM LGSFCACPPS FYGRNCEHDV RKENCGSVPH DTWLPKKCSL CKCWHGQLRC FPQAFLPGCD GLVMDEHLVA SRTPELPPS.

Biological Activity

In a functional ELISA, immobilized recombinant human Activin RIB/Fc chimera receptor (0.2 µg/mL) will bind TDGF1 with linear range of 0.8 to 100 ng/ml.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.