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Catalogue Number
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PRO-791
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Introduction
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Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
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Description
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Streptavidin Streptomyces Avidinii Recombinant produced in E.Coli.
The molecular weight per tetramer is approximately 53kDa.
Extinction coefficient per subunit: 41326 M-1 cm-1.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized in 10mM potassium phosphate buffer pH 6.5.
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Solubility
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It is recommended to reconstitute the lyophilized Streptavidin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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Stability
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Streptavidin is shipped at ambient temperature, upon arrival store at -20°C.
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Purity
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Greater than 95.0% as determined by SDS-PAGE.
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Specific Activity
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> 17U/mg (one unit binds 1 μg D-biotin at pH 8.9).
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Proteolytic Activity
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< 10-3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0).
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Usage
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ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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| Contact Us |
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Toll Free Phone: 1-(866)-245-0885
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Postal Address: PO Box 6591,
East Brunswick, 08816 NJ |
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Toll Free Phone: 00-800-800-66666
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International Phone: +972-8-9471175
International Fax: +972-8-9460534
Postal Address: PO Box 4157,
Ness-Ziona, 74140 Israel
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Website : www.prospecbio.com
Email: info@prospecbio.com |
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