Soybean P34
Soybean P34 Protein Recombinant

Introduction:
The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 2–3% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a ~32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons.

Description:
The E.Coli derived recombinant protein contains Soybean P34 Protein epitopes 214-261, 351-379 amino acids. The protein is fused to a GST tag at N-terminus.

Method: 
Purified by proprietary chromatographic technique.

Purity:
Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:
50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.

Storage:
Protein is shipped at ambient temperature.
Upon arrival, Store at -20°C

Stability:
Five years frozen. One month in solution at room temperature.

Applications:
Use as an antigen in ELISA and Western Blots.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.