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Catalogue Number
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ALR-002
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Introduction
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The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 2–3% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a ~32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons.
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Description
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The E.Coli derived recombinant protein contains Soybean P34 Protein epitopes 214-261, 351-379 amino acids. The protein is fused to a GST tag at N-terminus.
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Method
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Purified by proprietary chromatographic technique.
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Purity
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Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
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Formulation
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50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.
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Storage
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Soybean P34 although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
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Applications
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Use as an antigen in ELISA and Western Blots.
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Usage
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Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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Toll Free Phone: 1-(866)-245-0885
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Postal Address: PO Box 4157,
Ness-Ziona, 74140 Israel
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Website : www.prospecbio.com
Email: info@prospecbio.com |
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