ProSpec | Recombinant Protein Manufacturer
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Recombinant Proteins
A Disintegrin and Metalloproteinase Domain
(2 products) View Products
Actin
(7 products) View Products
Adaptor-Related Protein Complex
(3 products) View Products
ADP-Ribosylation Factor
(21 products) View Products
Advanced Glycosylation End Product-Specific Receptor
(2 products) View Products
Albumin
(6 products) View Products
Allergy
(18 products) View Products
Alpha-2-HS-Glycoprotein
(4 products) View Products
Ankyrin Repeat Domain
(5 products) View Products
Annexin
(15 products) View Products
Anterior Gradient Protein
(2 products) View Products
ASF1 Anti-Silencing Function 1
(2 products) View Products
Assembly Protein Complex
(0 products) View Products
ATP Synthase Mitochondrial
(3 products) View Products
ATPase
(6 products) View Products
Autophagy Related
(4 products) View Products
B Cell Lymphoma
(26 products) View Products
B9 Protein
(2 products) View Products
Babesia Microti
(2 products) View Products
Baculoviral IAP Repeat-Containing
(3 products) View Products
Basic Leucine Zipper Transcription Factor
(2 products) View Products
Basic Transcription Factor
(2 products) View Products
Bax
(2 products) View Products
Beta 2 Microglobulin
(3 products) View Products
BID
(4 products) View Products
Bromodomain Containing
(3 products) View Products
Cadherin
(5 products) View Products
Calbindin
(4 products) View Products
Calcium Binding Protein
(46 products) View Products
Calcyphosine
(2 products) View Products
Calmodulin
(4 products) View Products
Calpain
(2 products) View Products
Calponin
(2 products) View Products
Calreticulin
(2 products) View Products
Candida Albicans
(4 products) View Products
Canopy FGF Signaling Regulator
(3 products) View Products
Capping Protein
(2 products) View Products
Carcinoembryonic Antigen
(5 products) View Products
Cardiac Troponin
(13 products) View Products
Caspase Recruitment Domain Family
(2 products) View Products
CCAAT/enhancer binding protein
(2 products) View Products
CCR4-NOT Transcription Complex
(2 products) View Products
Cell Division Cycle
(8 products) View Products
Cellular Retinoic Acid Binding Protein
(2 products) View Products
Centrin
(3 products) View Products
Centromere Protein
(7 products) View Products
Charged Multivesicular Body Protein
(3 products) View Products
Chloride Intracellular Channel
(4 products) View Products
Chromatin Modifying Protein
(4 products) View Products
Chromobox
(3 products) View Products
Chromogranin
(4 products) View Products
Chromosome Open Reading Frame
(19 products) View Products
Clathrin
(2 products) View Products
Coagulation Factors
(6 products) View Products
Cofilin
(2 products) View Products
Coiled-Coil Domain
(9 products) View Products
Collagen
(5 products) View Products
COMM Domain Containing
(4 products) View Products
Complement Component
(22 products) View Products
COP9 Signalosome
(3 products) View Products
C-Reactive Protein
(4 products) View Products
Crystallin
(11 products) View Products
C-type Lectin Domain
(4 products) View Products
CUE Domain Containing
(2 products) View Products
Cystatin
(20 products) View Products
Cysteine-Rich
(2 products) View Products
Cysteine-Rich Secretory Protein
(3 products) View Products
Cytochrome
(7 products) View Products
Cytohesin
(4 products) View Products
Cytokeratin
(13 products) View Products
DCN1 Defective in Cullin Neddylation 1 Domain
(3 products) View Products
DEAD Box Protein
(2 products) View Products
Decorin
(3 products) View Products
Density Lipoprotein
(7 products) View Products
Developmental Pluripotency Associated
(3 products) View Products
Dickkopf-Related Protein
(5 products) View Products
DiGeorge Syndrome Critical Region
(2 products) View Products
DNA-Damage Protein
(7 products) View Products
Dynactin
(4 products) View Products
Dynein Light Chain
(9 products) View Products
Dysbindin
(2 products) View Products
Ectodysplasin
(2 products) View Products
Elongator Acetyltransferase Complex
(2 products) View Products
Endoplasmic Reticulum Protein
(3 products) View Products
EPH Receptor
(4 products) View Products
Ephrin
(8 products) View Products
Eukaryotic Translation Initiation Factor
(25 products) View Products
Excision Repair Cross-Complementing
(2 products) View Products
Exosome Component
(7 products) View Products
FABP
(21 products) View Products
Family with Sequence Similarity
(9 products) View Products
F-Box Protein
(2 products) View Products
Ferritin
(3 products) View Products
Fibrinogen
(3 products) View Products
Fibronectin Type III Domain Containing
(2 products) View Products
Four And A Half LIM
(2 products) View Products
Fragile Histidine Triad
(2 products) View Products
G Antigen
(3 products) View Products
GABA(A) Receptor-Associated Protein
(3 products) View Products
Gastrokine
(3 products) View Products
GDP Dissociation Inhibitor
(2 products) View Products
General Transcription Factor
(6 products) View Products
GIPC PDZ Domain
(2 products) View Products
Gliadin
(3 products) View Products
Glycoprotein Nmb
(2 products) View Products
Glypican
(2 products) View Products
Gremlin
(2 products) View Products
GTPase IMAP Family
(2 products) View Products
GTP-Binding Protein
(2 products) View Products
Guanine Nucleotide Binding Protein
(11 products) View Products
Hairy and Enhancer of Split
(2 products) View Products
Haptoglobin
(4 products) View Products
Heat Shock Protein
(66 products) View Products
Hematological And Neurological Expressed
(2 products) View Products
Hemoglobin
(7 products) View Products
Heterogeneous Nuclear Ribonucleoprotein
(4 products) View Products
High-Mobility Group
(8 products) View Products
Histidine Triad Nucleotide Binding Protein
(2 products) View Products
Homeobox
(9 products) View Products
Homer Homolog
(3 products) View Products
Hypoxia-Inducible Factor
(4 products) View Products
Immunoglobulin Heavy Chain Constant Region
(3 products) View Products
Inhibitor of DNA Binding
(2 products) View Products
Inhibitor of Growth Family
(2 products) View Products
Integrin
(3 products) View Products
Intercellular Adhesion Molecule
(4 products) View Products
Karyopherin
(2 products) View Products
Killer Cell
(5 products) View Products
Killer Cell Lectin-like Receptor
(4 products) View Products
Kirsten Rat Sarcoma Viral Oncogene
(2 products) View Products
Kruppel-Like Factor
(6 products) View Products
Lactoferrin
(3 products) View Products
Left-Right Determination Factor
(1 products) View Products
Leukocyte Cell Derived Chemotaxin
(3 products) View Products
Leukocyte-Associated Ig-Like Receptor
(3 products) View Products
LIN Protein
(4 products) View Products
Lipopolysaccarid Binding Protein
(2 products) View Products
Listeriolysin
(2 products) View Products
Lymphocyte Antigen
(3 products) View Products
Lysosomal-Associated Membrane Protein
(3 products) View Products
MAD2
(2 products) View Products
Mago-Nashi Homolog
(2 products) View Products
Major Histocompatibility Complex
(6 products) View Products
Maltose Binding Protein
(3 products) View Products
Mediator Complex
(5 products) View Products
Melanoma Antigen Family A
(8 products) View Products
Member RAS Oncogene Family
(47 products) View Products
Mesoderm Development Candidate
(3 products) View Products
Met Proto-Oncogene
(2 products) View Products
Methylmalonic Aciduria
(2 products) View Products
MHC class I chain-related gene
(3 products) View Products
Microfibrillar Associated Protein
(3 products) View Products
Microtubule-Associated Protein
(10 products) View Products
Mitochondrial Ribosomal Protein
(8 products) View Products
Mitochondrial Transcription Factor
(2 products) View Products
MOB1, Mps One Binder Kinase Activator
(2 products) View Products
Mortality Factor
(2 products) View Products
Mycobacterium Tuberculosis major secretory protein Antigen
(2 products) View Products
Myelin Basic Protein
(2 products) View Products
Myelin Oligodendrocyte Glycoprotein
(3 products) View Products
Myoglobin
(4 products) View Products
Myosin Light Chain
(15 products) View Products
Myxovirus
(3 products) View Products
NANOG
(4 products) View Products
NCK Adaptor Protein
(2 products) View Products
Nescient Helix Loop Helix
(2 products) View Products
Neuronal Calcium Sensor
(2 products) View Products
Neutrophil Cytosolic Factor
(2 products) View Products
NFKB Inhibitor
(5 products) View Products
NHP2
(2 products) View Products
N-Myc Downstream Regulated
(3 products) View Products
Non-Metastatic Cells
(4 products) View Products
NTF2-like Export Factor
(2 products) View Products
Nucleobindin
(3 products) View Products
Nucleophosmin
(2 products) View Products
Nucleopurin
(3 products) View Products
Nucleosome Assembly Protein
(2 products) View Products
Orosomucoid
(3 products) View Products
Other
(770 products) View Products
Outer Membrane Protein
(2 products) View Products
p53
(2 products) View Products
Paired Box
(2 products) View Products
Parkinson Disease Protein
(3 products) View Products
Parvalbumin
(2 products) View Products
PCNA
(3 products) View Products
PDZ Domain Containing
(2 products) View Products
Pentraxin
(2 products) View Products
Peroxisomal Biogenesis Factor
(2 products) View Products
PHD Finger Protein
(2 products) View Products
Phosducin-Like
(2 products) View Products
Phosphatase and Tensin
(2 products) View Products
Phosphatidylinositol Transfer Protein
(2 products) View Products
Phospholipid Scramblase
(2 products) View Products
PIH1 Domain Containing
(2 products) View Products
Pim Oncogene
(2 products) View Products
Polymerase (RNA) (DNA directed) Polypeptide
(7 products) View Products
Potassium Channel Tetramerisation Domain Containing
(4 products) View Products
POU Class
(4 products) View Products
Prefoldin
(6 products) View Products
Pregnancy Specific Beta-1-Glycoprotein
(2 products) View Products
Prion Protein
(2 products) View Products
Processing Of Precursor
(2 products) View Products
Profilin
(4 products) View Products
Programmed Cell Death
(5 products) View Products
Prohibitin
(2 products) View Products
Proprotein Convertase Subtilisin/Kexin
(1 products) View Products
Protein C-Ets
(2 products) View Products
Protein Phosphatase
(2 products) View Products
Protein-A, A/G & G
(24 products) View Products
Prothymosin
(2 products) View Products
RAD51
(3 products) View Products
RAN Binding Protein
(2 products) View Products
Ras Association Domain-Containing Protein
(2 products) View Products
Ras-Related C3 Botulinum Toxin Substrate
(5 products) View Products
Receptor Activity-Modifying Protein
(2 products) View Products
Regenerating Islet-Derived
(4 products) View Products
Regulator of Calcineurin
(3 products) View Products
Regulator of G-Protein Signaling
(10 products) View Products
Related RAS Viral (r-ras) Oncogene
(2 products) View Products
Relaxin
(2 products) View Products
Renin
(4 products) View Products
Reticulocalbin
(3 products) View Products
Retinoblastoma
(3 products) View Products
Retinoic Acid Early Transcript
(2 products) View Products
Retinoic Acid Receptor Responder
(3 products) View Products
Rho Family GTPase
(2 products) View Products
Rho GDP Dissociation Inhibitor
(2 products) View Products
Ribosomal Protein
(32 products) View Products
Ring Finger Protein
(5 products) View Products
RNA Binding Motif Protein
(4 products) View Products
R-Spondin
(1 products) View Products
RWD Domain Containing
(2 products) View Products
Sclerostin
(2 products) View Products
Secretagogin
(2 products) View Products
Secreted Frizzled-Related Protein
(3 products) View Products
Secretoglobin
(4 products) View Products
Secretogranin
(2 products) View Products
Selectin
(5 products) View Products
Selenoprotein
(3 products) View Products
Septin
(4 products) View Products
Serglycin
(2 products) View Products
Serine Peptidase Inhibitor
(3 products) View Products
Serpin
(23 products) View Products
SERTA Domain Containing
(2 products) View Products
SH3 Domain
(6 products) View Products
Signal Recognition Particle
(3 products) View Products
Signal Sequence Receptor
(3 products) View Products
Signal-Regulatory Protein
(3 products) View Products
Single-Stranded DNA Binding Protein
(2 products) View Products
Sirtuin
(5 products) View Products
SIX Homeobox
(2 products) View Products
SLAM Family
(5 products) View Products
SMAD
(3 products) View Products
Small Nuclear Ribonucleoprotein Polypeptide
(18 products) View Products
Solute Carrier Family
(2 products) View Products
Sorting Nexin
(3 products) View Products
SPRY Domain-Containing SOCS Cox Protein
(2 products) View Products
SRY (Sex Determining Region Y)-Box
(4 products) View Products
Stathmin
(4 products) View Products
Streptavidin
(5 products) View Products
Streptococcus Pyogenes
(2 products) View Products
Succinate Dehydrogenase Complex Assembly Factor
(2 products) View Products
Superoxide Dismutase
(7 products) View Products
Surfeit
(2 products) View Products
Synaptobrevin
(9 products) View Products
Synaptosomal Associated Protein
(6 products) View Products
Synaptotagmin
(6 products) View Products
Syndecan
(6 products) View Products
Synovial Sarcoma, X Breakpoint
(2 products) View Products
Syntaxin
(10 products) View Products
Synuclein
(12 products) View Products
Tachykinin
(2 products) View Products
TAR DNA
(3 products) View Products
TBC1 Domain Family
(2 products) View Products
T-cell Leukemia/Lymphoma
(2 products) View Products
Tetratricopeptide Repeat Domain
(3 products) View Products
THAP Domain
(4 products) View Products
Thioredoxin
(11 products) View Products
TIGAR
(2 products) View Products
Tissue Factor Pathway Inhibitor
(2 products) View Products
TNF receptor-Associated Factor
(2 products) View Products
Trafficking Protein Particle Complex
(3 products) View Products
Transcription Elongation Factor
(8 products) View Products
Transforming Growth Factor Beta Induced
(5 products) View Products
Transgelin
(3 products) View Products
Translocase Of Outer Mitochondrial Membrane
(2 products) View Products
Triggering Receptor Expressed on Myeloid Cells
(2 products) View Products
Tripartite Motif
(3 products) View Products
Tropomyosin
(4 products) View Products
TROVE Domain Family
(3 products) View Products
Trypsin
(4 products) View Products
Tubulin Folding Cofactor
(5 products) View Products
U6 Small Nuclear RNA
(6 products) View Products
Ubiquinol-Cytochrome C Reductase
(3 products) View Products
Ubiquitin
(24 products) View Products
UCHL1
(3 products) View Products
UL16 binding protein
(4 products) View Products
Uroplakin
(2 products) View Products
Vacuolar Protein Sorting
(7 products) View Products
Vascular cell adhesion molecule
(4 products) View Products
V-crk Sarcoma Virus CT10
(2 products) View Products
Vimentin
(3 products) View Products
Visinin-Like Protein
(3 products) View Products
V-maf Musculoaponeurotic Fibrosarcoma
(3 products) View Products
V-ral Simian Leukemia Viral Oncogene
(2 products) View Products
WAP Four-Disulfide Core Domain
(2 products) View Products
Y.Enterocolitica
(6 products) View Products
Zinc Finger
(16 products) View Products

About Recombinant Proteins:

Recombinant proteins are a new combination of genes that forms DNA. Recombinant DNA technology allows for the production of wild type and modified human and mammalian proteins at bulk quantities. Recombinant proteins are made from cloned DNA sequences which usually encode an enzyme or protein with known function

Recombinant proteins are made through genetic engineering, also called gene splicing or recombinant DNA technology. By putting human, animal or plant genes into the genetic material of bacteria, mammalian or yeast cells, these microorganisms can be used as factories or producers to make proteins for medical, academic and research uses.

A vector is simply a tool for manipulating DNA and can be viewed as a "transport vehicle" for the production of proteins from specific DNA sequences cloned into them. Purification and expression of a protein can sometimes be quite complicated & time-consuming, therefore an additional tag is used in addition to the specific DNA sequence which will facilitate the purification & expresion of the recombinant protein.

Recombinant Proteins are proteins that their DNA that has been created artificially. DNA from 2 or more sources which is incorporated into a single recombinant molecule. The DNA is first treated with restriction endonuclease enzyme which the ends of the cut have an overhanging piece of single-stranded DNA. These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end. DNA ligase covalently links the two strands into 1 recombinant DNA molecule.

Recombinant DNA molecule must be replicated many times to provide material for analysis & sequencing. Producing many identical copies of the same recombinant DNA molecule is called cloning. Cloning is done in vitro, by a process called the polymerase chain reaction (PCR). Cloning in vivo can be done in unicellular microbessuch as E. coli, unicellular eukaryotes like yeast and in mammalian cells grown in tissue culture.

Recombinant DNA must be taken up by the cell in a form in which it can be replicated and expressed. This is achieved by incorporating the DNA in a vector. A number of viruses (both bacterial and of mammalian cells) can serve as vectors.

Recombinant DNA is also sometimes referred to as chimera. When combining two or more different strands of DNA.There are 3 different methods by which Recombinant DNA is made. 1. Transformation, 2. Phage-Transfection 3.Yeast, Plant & Mammalian Transformation. When using the method of transformation one needs to select a piece of DNA to be inserted into a vector, cut a piece of DNA with a restriction enzyme and ligate the DNA insert into the vector with DNA Ligase. The insert contains a selectable marker which allows for identification of recombinant molecules. An antibiotic marker is used in order to cause death for a host cell which does not contain the vector when exposed to a certain antibiotic.
Trasnformation is the insertion of the vector into the host cell. The host cells are prepared to take up the foreign DNA. Selectable markers are used for antibiotic resistance, color changes, or any other characteristic which can distinguish transformed hosts from untransformed hosts. Yeast, Plant & Mammalian Transformation is done by micro-injecting the DNA into the nucleus of the cell being transformed. Phage-Transfection process, is equivalent to transformation except for the fact that phage lambda or MI3 is used instead of bacteria.
These phages produce plaques which contain recombinant proteins which can be easily distinguished from the non-recombinant proteins by various selection methods.

Significant amounts of recombinant protein are produced by the host only when expression genes are added. The Protein’s expression depends on the genes which surround the DNA of interest, this collection of genes act as signals which provide instructions for the transcription and translation of the DNA of interest by the cell. These signals include the promoter, ribosome binding site, and terminator.

The recombinant DNA is inserted into expression vectors which contain the promoter, ribosome binding site, and terminator.
In prokaryotic systems, the promoter, ribosome binding site, and terminator have to be from the same host since the bacteria is unlikely to understand the signals of human promoters and terminators. The designated gene must not contain human introns since the bacteria does not recognize it and this results in premature termination, and the recombinant protein may not be processed correctly, be folded correctly, or may even be degraded.

The peptide sequence can be added as an extension at the N-terminal. Researchers can select the specific purification system which they would like to use. The unique vectors available contain several features needed for the production of bulk quantities of the target protein. The peptide sequence is usually placed in the vector so that it is designed to be a point of attack for a specific protease. Thus, after the recombinant protein is expressed and extracted from bacteria, specific peptide extension can be used to purify the protein and subsequently removed from the target protein to generate a nearly natural sequence on the final product.

6 or more consistent Histidine residues act as a metal binding site for recombinant protein purification and expression. The hexa-His sequence is called a His-Tag sequence which can be placed on the N-terminal of a target protein by using vectors from various commercial molecular biology companies. The His-Tag contains a cleavage site for a specific protease. His-Tag recombinant proteins are purified by Metal Chelate Affinity Chromatography such as nickel ion columns that are used as the heavy metal ion and the His-Tag protein is eluted from the metal-chelate column with Histidine or imidazole. Then the purified His-Tag protein is treated with the specific protease to cleave off the His-Tag or not if the tag doesn’t affect the active site of the protein.

Proteins have metal binding sites which can be used for the purification of recombinant and natural proteins. This type of purification is rather simple when using a gel bead which is covalently modified so that it displays a chelator group for binding a heavy metal ion like Ni2+ or Zn2+. The chelating group on the gel bead contains a small amount of the ligands needed to hold the metal ion. So when the protein’s metal binding site finds the heavy metal, it will bind by providing the ligands from its metal binding site to attach to the metal ion displayed on the chelator location of the gel bead. This purification method is quite identical to affinity chromatography when purifying metal-binding class of proteins.

Contact Us

Toll Free Phone: 1-(866)-245-0885
Toll Free Fax:  1-(866)-796-4944
Postal Address: PO Box 6591,
East Brunswick, 08816 NJ

Toll Free Phone: 00-800-800-66666
Toll Free Fax: 00-800-800-67890

International Phone: +972-8-9471175
International Fax: +972-8-9460534
Postal Address: PO Box 4157,
Ness-Ziona, 74140 Israel

Website : www.prospecbio.com
Email: info@prospecbio.com

 
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