Polyphosphate Kinase

Description

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Quantity

Shipping

	50IU($650)
	100IU($1100)
	200IU($2000)

Catalogue Number

ENZ-266

Synonyms

Polyphosphate kinase, EC 2.7.4.1, Polyphosphoric acid kinase, ATP-polyphosphate phosphotransferase.

Introduction

PPK catalyzes the reversible transfer of phosphate between polyphosphate and ATP. The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction.

Description

Polyphosphate Kinase purified circa 10 fold . Free of all Polyphosphate Glucokinase activity.
Native MW = 83 kD (monomer).

Source

Propionibacterium shermanii.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from 1.15ml PPK solution containing 43.6 U/ml of PPK activity, 0.77 mg/ml total protein, 10mM potassium phosphate pH 6.8 and 25mM sodium polyphosphate.

Solubility

The contents of the vial can be reconstituted in 1-10 ml deionized water.

Stability

Lyophilized Polyphosphate kinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PPK should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Definition of Activity Units

The amount of Polyphosphate kinase required to convert 1 µmole ADP to ATP per minute at pH 7.5, using polyphosphate as phosphate donor.

Specific Activity

56.5 U/mg.

Required Cofactors

The reaction requires Mg2+.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.