Phospholipase A2

Description

Our Price

Quantity

Shipping

Phospholipase A2 P00630 Bee Venom Protein Recombinant

	100µg($150)
	500µg($600)
	1000µg($1000)

Ice Packs

Catalogue Number

ALR-001

Synonyms

Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Allergen Api m I, Api m 1.

Introduction

Bee venom phospholipase A2 (PLA) is the main allergen in the bee sting allergy. Bee venom phospholipase A2 (BV-PLA2) is a hydrolytic enzyme which specifically cleaves the sn-2 acyl bond of phospholipids at the lipid/water interface. BV-PLA2 is a 14–16-kDa glycoprotein, consisting of 134 amino acids and displaying a single carbohydrate side chain at the residue Asn13. It is also held to be responsible for some systemic anaphylactic reactions in bee venom sensitized individuals. BV-PLA2 presents 3 peptide and a glycopeptide T cell epitopes, which are recognized by both allergic and non-allergic bee venom sensitized subjects. PLA is able to elicit both IgE mediated allergy and normal immunity to bee sting which usually is associated with high affinity IgG4 anti-PLA antibodies.

Description

The E.Coli derived recombinant protein contains phospholipase P00630 bee venom protein epitopes, 26-162 amino acids.

Method

Purified by proprietary chromatographic technique.

Purity

Protein is >90% pure as determined by 10% SDS-PAGE.

Formulation

20mM Tris-HCl, pH 7.2, 1.5M urea and 50% glycerol.

Storage

Phospholipase A2 although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.

Applications

:
Use as an antigen in ELISA and Western Blots.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.