PRKCB2 Human | ProSpec

Description

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Protein Kinase C beta II Human Recombinant

	2µg($50)
	10µg($130)
	1mg($4800)

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Catalogue Number

PKA-319

Synonyms

Protein kinase C beta type, EC 2.7.11.13, PKC-beta, PKC-B, PKCB, PRKCB, PRKCB2, MGC41878.

Introduction

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Description

PKC-bII Human Recombinant produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-673 and having a molecular mass of 80 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag.
PKC-bII is purified by proprietary chromatographic techniques.

Source

Sf9 insect cells.

Formulation

PKC-bII is supplied at a of 0.1mg/ml in 10mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.

Unit Definition

780 units/mg. One unit of PKC??? activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30°C. Recombinant human active PKC??? also phosphorylates full length
myelin basic protein, histone, HIV Tat-1, GSK-3? and cdk7. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.