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Catalogue Number
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ENZ-103
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Synonyms
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Calcineurin subunit B type 2, Calcineurin B-like protein, CBLP, Calcineurin BII, CNBII, PPP3R1-like, Protein phosphatase 2B regulatory subunit 2, Protein phosphatase 3 regulatory subunit B beta isoform, PPP3R2, PPP3RL.
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Introduction
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PPP3R2 is a calcium-dependent, calmodulin stimulated serine/threonine protein phosphatase. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is a crucial means of regulating a wide range of cellular functions including division, homeostasis and apoptosis. Ordinarily the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit.
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Description
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PPP3R2 Human Recombinant fused with a 20 a.a. His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a. 1-173) containing 193 amino acids and having a molecular mass of 22kDa.
The PPP3R2 is purified by proprietary chromatographic techniques.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered colorless solution.
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Formulation
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The PPP3R2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol and 1mM DTT.
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Stability
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Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
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Purity
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Greater than 95.0% as determined by SDS-PAGE.
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Amino acid sequence
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MGSSHHHHHH SSGLVPRGSH MSTMGNEASY PAEMCSHFDN DEIKRLGRRF KKLDLDKSGS LSVEEFMSLP ELRHNPLVRR VIDVFDTDGD GEVDFKEFIL GTSQFSVKGD EEQKLRFAFS IYDMDKDGYI SNGELFQVLK MMVGNNLTDW QLQQLVDKTI IILDKDGDGK ISFEEFSAVV RDLEIHKKLV LIV.
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Usage
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ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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