POU domain class 5 transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3, OTF4, MGC22487.
POU5F1 is a homeodomain transcription factor of the POU family, expressed in embryonic stem (ES) cells and embryonic carcinoma (EC) cells.POU5F1 is significantly involved in the signaling pathway for maintaining self-renewal and pluripotency of ES cells. POU5F1 has 2 distinct DNA binding domains which independently bind half-sites of the canonical octamer motif. This flexibility allows POU5F1 to bind with distinct DNA motifs by forming heterodimers with other transcription factors or by forming homodimers in several conformations. Human POU5F1 contains a 75aa POU specific (POUS) domain and a 60aa POU-Homeo-(POUH) domain connected by a linker region. The Human POU5F1 specifically interacts with Octamer motif ATGCAAAT. In addition, 2 proline-rich domains in the N-terminal and C-terminal regions are vital for POU5F1 transactivation. POU5F1 regulates a number of target genes and has been shown to work jointly with other transcription factors including Sox2 as well as Nanog to sustain stem cell potency and self-renewal.
POU5F1 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 360 amino acids and having a molecular mass of 38.6kDa.The POU5F1 is fused to an N-terminal TAT (transcriptional activator protein) tag and purified by proprietary chromatographic techniques.
POU5F1 was lyophilized after extensive dialysis against PBS.
It is recommended to reconstitute the lyophilized POU5F1 in sterile PBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gly-Arg-Lys-Lys.
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