- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-501
Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide.
Introduction
PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.
Description
Platelet-Derived Growth Factor BB Human Recombinant is a homodimeric, non-glycosylated, polypeptide chain containing 2x109 amino acids (218 amino acids in total) and having a molecular mass of 24.3 kDa. PDGF-BB is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4.
Solubility
It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT.
Biological Activity
Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells.
The expected ED50 for this effect is 1.0-3.0 ng/ml.
The expected ED50 for this effect is 1.0-3.0 ng/ml.
References
1.Title:Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathway.
Publication:Published online before print October 30, 2009, doi: 10.1182/blood-2009-06-223719 Blood January 7, 2010 vol. 115 no. 1 51-60 .
Link:PDGF BB prospec publication
2.Title: attenuates skeletal muscle dystrophy in mdx mice.
Publication:Published online before print March 16, 2009, doi: 10.1096/fj.09-129833 August 2009 The FASEB Journal vol. 23 no. 8 2539-2548.
Link:Platelet-Derived Growth Factor BB prospec publication
3. Title:Human Cord Blood-Derived Endothelial Progenitor Cells and Their Conditioned Media Exhibit Therapeutic Equivalence for Diabetic Wound Healing.
Publication: Cell Transplantation, Vol. 19, pp. 1635–1644, 2010 0963-6897/10 $90.00 + .00
Printed in the USA. All rights reserved. DOI: 10.3727/096368910X516637
Copyright ? 2010 Cognizant Comm. Corp.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
Recombinant Human BB Platelet-Derived Growth Factor (PDGF-BB): Multifaceted Roles in
Development, Tissue Repair, and Disease Progression
Abstract:
Platelet-Derived Growth Factor BB (PDGF-BB), also known as ODGF, SIS, SSV, PDGF-BB, or PDGF B-chain, is a potent growth factor involved in numerous physiological and pathological processes.
This research paper aims to comprehensively explore the multifaceted roles of recombinant human PDGF-BB in development, tissue repair, and disease progression. Through an in-depth analysis of its molecular characteristics, signaling pathways, and diverse physiological functions, this paper provides insights into the significance of PDGF-BB as a
critical regulator in various biological contexts.
Introduction :
Platelet-Derived Growth Factor BB (PDGF-BB) is a growth factor that plays a pivotal role in diverse biological processes, including embryonic development, tissue repair, and disease progression. This section provides an introduction to PDGF-BB and its synonyms, such as ODGF, SIS, SSV, PDGF-BB, and PDGF B-chain, highlighting their relevance and significance in scientific literature.
Molecular Characteristics of PDGF-BB:
This section explores the molecular characteristics of PDGF-BB, including its primary amino acid sequence, protein structure, post-translational modifications, and binding partners. The importance of these factors in determining PDGF-BB's biological activity, receptor binding affinity, and downstream signaling pathways is discussed in detail.
Signaling Pathways Activated by PDGF-BB:
PDGF-BB engages specific signaling pathways upon binding to its receptors, leading to diverse cellular responses. This section focuses on the canonical PDGF receptor (PDGFR) signaling cascade, highlighting the activation of Ras/MAPK, PI3K/Akt, and PLCγ pathways. The downstream effectors and transcription factors involved in mediating PDGF-BB's cellular responses are also discussed.
Physiological Functions of PDGF-BB:
PDGF-BB plays crucial roles in various physiological processes, including embryonic development, angiogenesis, tissue repair, and homeostasis. This section examines PDGF-BB's contributions to cell proliferation, migration, differentiation, and survival, emphasizing its effects on specific cell types and tissues.
PDGF-BB in Disease Progression:
PDGF-BB is implicated in the progression of several diseases, including cancer, fibrosis, and vascular disorders. This section delves into PDGF-BB's involvement in tumor growth, angiogenesis, metastasis, tissue fibrosis, and vascular remodeling. The therapeutic implications and targeting of PDGF-BB in disease management are also discussed.
Clinical Applications and Future Perspectives:
Recombinant human PDGF-BB holds significant clinical potential for tissue repair, wound healing, and regenerative medicine. This section highlights the current applications of PDGF-BB in clinical settings, including bone and soft tissue repair, chronic wound management, and periodontal regeneration. Furthermore, it discusses ongoing research efforts and potential future directions in utilizing PDGF-BB for therapeutic purposes.
Conclusion :
Recombinant human PDGF-BB, also known as ODGF, SIS, SSV, PDGF-BB, or PDGF B-chain, plays multifaceted roles in development, tissue repair, and disease progression. Through its molecular characteristics, signaling pathways, and diverse physiological functions, PDGF-BB emerges as a critical regulator in various biological contexts.