PCNA

PCNA

PCNA

Name

Description

Product #

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Quantity

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  • View Data Sheet

    Description:

    Proliferating Cell Antigen Human Recombinant, Sf9

    Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.

    Product # :

    PRO-303

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    • description
    • source
    • formulation
    • purity
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    Description

    PCNA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 35 kDa. PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

    Source

    Sf9 insect cells.

    Formulation

    Recombinant Human PCNA is supplied in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      Proliferating cell nuclear antigen (PCNA), a protein of 30 kDa molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA replication fork. PCNA thereby assumes important functions in DNA synthesis, repair and processing, the regulation of cell cycle and even apoptosis.
      Anti-PCNA antibodies occur in systemic lupus, where they represent a rare specificity (< 10 % of SLE patients). However, their presence alone or in combination with other autoantibodies should alert the clinician to the presence of defined or evolving SLE.

    • Synonyms

      Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

    • Immunological Functions

      1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera.

    • coating concentration

      0.5-1.1 µg/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

    • Applications

      Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Pcna Human Sf9
  • View Data Sheet

    Description:

    Proliferating Cell Nuclear Antigen Human Recombinant

    Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.

    Product # :

    PRO-615

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    Quantity :

    Shipping Method :

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    • description
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    Description

    PCNA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 261 amino acids (1-261 a.a) and having a molecular mass of 28.7kDa. PCNA is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The PCNA solution contains 20mM Tris pH-7.5 & 2mM EDTA & 20% glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      PCNA is located in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps elevate the processivity of leading strand synthesis during DNA replication. In reaction to DNA damage, PCNA is ubiquitinated and takes part in the RAD6-dependent DNA repair pathway. 2 transcript variants encoding the same protein have been found for PCNA gene. Pseudogenes of this PCNA gene have been described on chromosome 4 and on the X chromosome. PCNA is expressed during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA is induced by UV irradiation.

    • Synonyms

      Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQLTLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Pcna Human

About PCNA / Proliferating Cell Nuclear Antigen Protein:

Cell nuclear antigen protein PCNA is a DNA polymerase that helps initiate the synthesis of DNA. It also assists in both DNA repair and replication processes.
PCNA is a nucleotide-binding protein with three domains:
●An N-terminal region for ATP binding
●A middle part responsible for catalysis
●The C-terminus which binds to DNA
This enzyme can be in various locations throughout the cell, such as chromatin during mitosis or after UV irradiation.

Mechanism of PCNA
PCNA binds to the single-stranded DNA on one side and, with ATP present, it helps direct the second strand of DNA. PCNA also facilitates the removal of RNA primers in replication and is involved in maintaining fidelity during replication.
This protein is essential for genome stability and sensitivity to UV irradiation as well as for DNA repair.

Functions of Proliferating Cell Nuclear Antigen Protein
PCNA has an extensive range of functions in the cell and is involved with processes such as replication, transcription, recombination, and meiosis.
It helps maintain genome stability by repairing single-stranded breaks that can lead to mutations or rearrangements. Cells need an increased PCNA level when exposed to UV-light or cisplatin.
PCNA is a vital component of the DNA replication process; it interacts with the primase complex to form an elongating RNA primer and helps maintain fidelity during replication by removing RNA primers from newly synthesized strands of DNA before they copy.
It also assists in recombination reactions that produce genetic diversity, and it is involved in meiosis and mitosis.
In conclusion, PCNA is a protein that helps initiate the synthesis of DNA. It assists in both DNA repair and replication processes, as well as maintaining genome stability.