NGB Human
Neuroglobin Human Recombinant

Synonyms:
NGB.

Introduction:
Neuroglobin, 151 amino acid residue protein, mainly expressed in vertebrate brain and retina, is a recently identified member of the globin superfamily. Augmenting O (2) supply, neuroglobin promotes survival of neurons upon hypoxic injury, potentially limiting brain damage. Moreover, neuroglobin may be a novel oxidative stress-responsive sensor for signal transduction in the brain. Neuroglobin expression is increased by neuronal hypoxia in vitro and focal cerebral ischemia in vivo, and neuronal survival after hypoxia is reduced by inhibiting neuroglobin expression with an antisense oligodeoxynucleotide and enhanced by neuroglobin overexpression.

Description:
17kDa protein containing 151 amino acid residues of the Neuroglobin human
MERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSFSTVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDG E

Specificity:
The amino acid sequence of the Neuroglobin human recombinant is 100% homologous to the amino acid sequence of the human Neuroglobin.

Purification Method:
Two-step procedure using size exclusion chromatography before and after refolding.

Source:
Escherichia Coli.

Purity:
>95% (SDS-PAGE analyzed).

 Formulation:
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer, 0.1M NaCl, pH 7.2.

Reconstitution:
Add 0.2 ml of H₂O and let the lyophilized pellet dissolve completely.

Storage:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Stability/Shelf Life:
The lyophilized protein remains stable until the expiry date when stored at -20°C.

Applications:
Western blotting.

References:
• Pesce A, Dewilde S, Nardini M, Moens L, Ascenzi P, Hankeln T, Burmester T, Bolognesi M. Human brain neuroglobin structure reveals a distinct mode of controlling oxygen affinity. Structure (Camb). 2003 Sep; 11(9): 1087-95.

• Garry DJ, Mammen PP. Neuroprotection and the role of neuroglobin. Lancet. 2003 Aug 2; 362(9381): 342-3.

• Sun Y, Jin K, Peel A, Mao XO, Xie L, Greenberg DA. Neuroglobin protects the brain from experimental stroke in vivo. Proc Natl Acad Sci U S A. 2003 Mar 18; 100(6): 3497-500. Epub 2003 Mar 05.

• Mammen PP, Shelton JM, Goetsch SC, Williams SC, Richardson JA, Garry MG, Garry DJ. Neuroglobin, a novel member of the globin family, is expressed in focal regions of the brain. JHistochem Cytochem. 2002 Dec; 50(12): 1591-8.

• Sun Y, Jin K, Mao XO, Zhu Y, Greenberg DA. Neuroglobin is up-regulated by and protects neurons from hypoxic-ischemic injury. Proc Natl Acad Sci U S A. 2001 Dec 18; 98(26): 15306-11. Epub 2001 Dec 11.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.