NAPA Human | ProSpec

Description

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Quantity

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N-Ethylmaleimide-Sensitive Factor Attachment Protein, Alpha Human Recombinant

	5µg($50)
	20µg($130)
	1mg($2700)

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Catalogue Number

PRO-250

Synonyms

SNAPA, SNAP-alpha.

Introduction

NAPA is part of the SNAP (Soluble NSF Attachment Protein) family. SNAPs, acting together with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are necessary for the fusion of transport vesicles to their objective membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. NAPA is in charge of the binding of NSF and therefore the formation of a 20S fusion particle.

Description

NAPA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 315 amino acids (1-295) and having a molecular mass of 35.3 kDa. NAPA is fused to 20 amino acid His Tag at N-terminus and purified by standard chromatography techniques.

Source

Escherichia Coli.

Physical Appearence

Sterile Filtered colorless solution.

Formulation

NAPA protein solution (1mg/ml) contains 20mM Tris-HCl pH-7.5 and 10% glycerol.

Stability

NAPA although stable 4°C for 4 weeks, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA AGNAFCQAAQ
LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS
ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA
HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.