NANOG

NANOG

NANOG

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  • View Data Sheet

    Description:

    NANOG-TAT Human Recombinant

    NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.

    Product # :

    PRO-090

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    • description
    • source
    • formulation
    • purity
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    Description

    NANOG Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 304 amino acids and c-terminal 13 amino acid TAT peptide having a molecular mass of 36.1kDa.The NANOG is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    NANOG protein solution contains PBS and 50mM Arginine.

    Purity

    Greater than 95.0% as determined by SDS-PAGE analysis.

    More Info

    • Introduction

      NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.

    • Synonyms

      NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.

    • Physical Appearance

      Sterile Filtered solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      VDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDVGGYGRK KRRQRRR

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Nanog Tat Human
  • View Data Sheet

    Description:

    Nanog Homeobox Pseudogene 8 Human Recombinant

    Nanog Homeobox Pseudogene 8, NANOG, NANOGP1, PN8.

    Product # :

    PRO-1553

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    Description

    NANOGP8 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 305 amino acids (1-305) and having a molecular mass of 34.6kDa.NANOGP8 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The NANOGP8 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 5% glycerol.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      NANOGP8 belongs to the homeobox family of DNA binding transcription factors which is known to conserve pluripotency of embryonic stem cells. One change only separate NANOGP8 from NANOG – instead of 'Gln-253' in the inferred amino acid sequence in NANOG there is a ‘His-253’ in NANOGP8.

    • Synonyms

      Nanog Homeobox Pseudogene 8, NANOG, NANOGP1, PN8.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA ENSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMHFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDV

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Nanogp8 Human
  • View Data Sheet

    Description:

    Nanog Human Recombinant, His Tag

    NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.

    Product # :

    PRO-881

    Price :

    Quantity :

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    • description
    • source
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    Description

    NANOG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 174 amino acids (1-154) and having a molecular mass of 19.6 kDa.The NANOG is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    NANOG protein (0.25mg/ml) is supplied in 20mM Tris-HCL, pH-8, 1mM DTT and 20% Glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.

    • Synonyms

      NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQ

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Nanog Human His
  • View Data Sheet

    Description:

    NANOG Human Recombinant

    NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.

    Product # :

    PRO-896

    Price :

    Quantity :

    Shipping Method :

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    Shipped with Ice Packs

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    More Info

    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    NANOG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 305 amino acids (1-305) and having a molecular mass of 34.6 kDa.The NANOG is purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The NANOG solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 5% glycerol.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.

    • Synonyms

      NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDV

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Nanog Human

About NANOG:

NANOG Function
NANOG is a transcription factor in embryonic stem cells. It is believed to be a key factor in maintaining pluripotency in cells. The reason that these cells are so important is because of their ability to maintain pluripotency. What this means is that they are able to become pretty much any cell of any of the three germ layers.
NANOG has also been known to repress the expression of the transcription factor Tal1 which inhibits embryonic hematopoiesis. It is also known to have expressed pluripotency marker genes that allowed for the differentiation in vitro and in vivo conditions into the other derivatives.
Ectopic expression of NANOG in adult stem cells can restore the proliferation and differentiation potential that is lost due to organismal aging.

NANOG Structure
The human NANOG protein consists of 305 amino acids and has three functional domains. It has the N-terminal domain, the C-terminal domain and the conserved homeodomain motif. The latter of these facilitated DNA binding. The NANOG 1 gene can be found on chromosome 12 which has a 915 bp open reading frame with 4 econs and 3 introns.
There are additional NANOG genes that are thought to potentially impact ESC’s differentiation.

NANOG Interaction
It is thought to function together with other factors such as POU5F1 and SOX2 in order to establish ESCF identity.

NANOG Mechanism
NANOG has the same mechanisms that maintain a cell’s pluripotency as this is one of the very things that does it. Overall, NANOG is extremely important for pluripotency. The stem cells that are pluripotent are able to form any terminally differentiated cell.