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Stromelysin-1, EC 3.4.24.17, Matrix metalloproteinase-3, MMP-3, Transin-1, SL-1, STMY, STR1, STMY1, MGC126102, MGC126103, MGC126104.
MMP-3 enzyme is also known as Stromelysin-1or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk . MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta.
Matrix Metalloproteinase-3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 230 amino acids and having a molecular mass of 22 kDa.
The MMP-3 is purified by proprietary chromatographic techniques.
Escherichia Coli.
Sterile Filtered White lyophilized (freeze-dried) powder.
The protein was lyophilized from a concentrated (1 mg/ml) solution with no additives.
It is recommended to reconstitute the lyophilized Stromelysin-1 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Matrix Metalloproteinase-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MMP-3 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Greater than 98% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
The specific activity was found to be ≥1,250 mU/mg protein (H. Nagase, et al; J. Biol. Chem. 269, 20952, 1994).
One unit is defined as the amount of enzyme thathydrolyzes 1µmol of MCA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH2 per min. at 37°C, pH 7.0.
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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