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Small inducible cytokine A2, CCL2, Monocyte chemotactic protein 1, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte secretory protein JE, HC11, chemokine (C-C motif) ligand 2, MCP1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, GDCF-2 HC11.
Mouse Anti Human Monoclonal
Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokinebelonging to the CC chemokinefamily that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclastsand osteoblastsand is under the control of nuclear factor κB (NFκB). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursorcontaining signal peptide of 23 amino acidsand a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weightof approximately 13kDa. As with many other CC chemokines, CCL2 is located on chromosome 17in humans. The cell surface receptors that bind CCL2 are CCR2and CCR5.
YNR-HMCAF
r.Human MCAF
Mouse IgG2a
Ion exchange.
1mg/ml in PBS (after reconstitution)
Antibody is shipped lyophilized at ambient temperature.
In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.
Two years lyophilized, one month in solution at 4oC.
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories) 1:5,000 dilution will give 0.3 O.D within 10 minutes.
Direct ELISA, Western Blot, Immuneprecipitation.
MCAF does not bind to plastic as good as most proteins - use higher concentration for proper binding.
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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