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Protein Catalog >> Enzymes >> Mitogen-Activated Protein Kinase  >> MAPK3 Human, His
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Description
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Mitogen-Activated Protein Kinase 3 Human Recombinant, His-Tag
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Catalogue Number PKA-265
Synonyms ERK1, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAP kinase3.
Introduction MAPK3 is a part of the MAP kinase family which is recognized as extracellular signal-regulated kinases (ERKs), that function in a signaling cascade that controls various cellular procedures such as proliferation, differentiation, and cell cycle progression in reaction to a variety of extracellular signals. MAPK3 is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.
Description MAPK3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 399 amino acids (1-379 a.a.) and having a molecular mass of 45.2 kDa. The MAPK3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearence Sterile Filtered colorless solution.
Formulation The MAPK3 0.5mg/ml protein solution contains 20 mM Tris-HCl buffer pH-8, 1mM DTT, 0.1M NaCl and 20% glycerol.
Stability MAPK3 although stable 4C for 4 weeks, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MAAAAAQGGG GGEPRRTEGV GPGVPGEVEM VKGQPFDVGP RYTQLQYIGE GAYGMVSSAY DHVRKTRVAI KKISPFEHQT YCQRTLREIQ ILLRFRHENV IGIRDILRAS TLEAMRDVYI VQDLMETDLY KLLKSQQLSN DHICYFLYQI LRGLKYIHSA NVLHRDLKPS NLLINTTCDL KICDFGLARI ADPEHDHTGF LTEYVATRWY RAPEIMLNSK GYTKSIDIWS VGCILAEMLS NRPIFPGKHY LDQLNHILGI LGSPSQEDLN CIINMKARNY LQSLPSKTKV AWAKLFPKSD SKALDLLDRM LTFNPNKRIT VEEALAHPYL EQYYDPTDEP VAEEPFTFAM ELDDLPKERL KELIFQETAR FQPGVLEAP.
Usage ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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