|
OB Protein, Obesity Protein, OBS, Obesity factor.
Leptin Binding Domain Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa.
The Leptin Binding Domain is purified by proprietary chromatographic techniques.
Escherichia Coli.
Sterile Filtered White lyophilized (freeze-dried) powder.
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
The lyophilized Leptin Binding Domain is very soluble in water and most aqueous buffers below and above the isoelectric point.
Lyophilized Leptin Binding Domain although stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C.
Please prevent freeze-thaw cycles.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
The sequence of the first four N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val
Biological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
|
