JO 1 Human Sf9 | ProSpec

Description

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Histidyl-tRNA Synthetase Human Recombinant, Sf9

	5µg($50)
	25µg($130)
	1mg($3900)

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Catalogue Number

ENZ-335

Synonyms

Histidyl-tRNA synthetase, EC 6.1.1.21, Histidine-tRNA ligase, HisRS, HRS, FLJ20491, JO-1.

Introduction

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Description

Histidyl-tRNA Synthetase Human Recombinant produced in baculovirus is a single, glycosylated, polypeptide chain having a molecular mass of 58.3 kDa.
The Histidyl-tRNA Synthetase is fused to 6x His Tag and purified by proprietary chromatographic techniques.

Source

Sf9 Insect Cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The protein solution contains 20mM HEPES, 250mM sodium chloride 0.1% and 20% Glycerol, (pH 7.5).

Stability

Histidyl-tRNA Synthetase although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Protein content

Protein quantitation was carried out by using 0.25 - 2.0 mg/ml Bradford assay vs. BSA.

Western Blot

Strongly reactive with human anti Histidyl-tRNA Synthetase antisera.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.