Isocitrate Dehydrogenase

Description

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Isocitrate Dehydrogenase (NADP +) Recombinant

	1mg($50)
	5mg($130)
	50mg($980)

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Catalogue Number

ENZ-289

Synonyms

Isocitrate dehydrogenase [NADP] cytoplasmic, EC 1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, IDH, NADP(+)-specific ICDH, IDP, PICD.

Description

Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
The ICDH is purified by proprietary chromatographic techniques.

Source

Saccharomyces Cerevisiae.

Physical Appearance

Sterile Filtered clear solution.

Formulation

One ml of solution (1mg/340µl) contains 0.075 mol/l KPO₄, 50% Glycerol, pH 7.1.

Stability

ICDH although stable at 15°C for 1 week should be stored between
2°C-8°C.
For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA)
Please avoid freeze-thaw cycles.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity

The specific activity was found to be 119 U/mg.

Unit Definition

One unit is defined as 1 ?mol of NAD+ production per minute under the assay conditions (25°C, pH 7.5).

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.