Interleukin 2 Mouse His

Description

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Quantity

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Interleukin-2 Mouse Recombinant, His Tag

	10µg($50)
	50µg($130)
	1mg($1800)

Ice Packs

Catalogue Number

CYT-624

Synonyms

Interleukin-2, T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2, Il2.

Introduction

IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

Description

Interleukin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 170 amino acids (21-169 a.a) and having a molecular mass of 19.5kDa.
The mouse IL-2 is fused to a 20 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless clear solution.

Formulation

The Mouse IL2 protein (0.5mg/ml) is supplied in Phosphate Buffered Saline (pH7.4) 0.1M NaCl, and 20% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Biological activity

Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 2.5 ng/ml, corresponding to a specific activity of less than 400,000units/mg.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF CQSVISTSPQ.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.