IL-2 Antibody
Interleukin-2, Mouse Anti-Human

Synonyms:
T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Type:
Mouse Anti Human Monoclonal

Introduction:
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

Immunogen:
r.Human IL-2

Ig Subclass:
Mouse IgG2a

Purification Method:
Ion Exchange

Protein concentration:
1mg/ml in PBS (after reconstitution)

Shipping Conditions:
Antibody is shipped lyophilized at ambient temperature.

Storage Procedures:
In lyophilized form, for long periods, store at 4^oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20^oC.

Reconstitution:
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Stability / Shelf Life:
Two years lyophilized, one month in solution at 4^oC.

Titer:
By direct ELISA: 1:10,000 dilution will yield 0.4 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). 1:50 dilution will block 70-80% of CTLL proliferative response to 50 units of r.human IL-2.

Applications:
Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry, blocking T cell poroliferation.

Cross reactivity:
A certain degree of functional cross reactivity with murine IL-2 was reported by some investigators.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.