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Protein Catalog >> Monoclonal Antibodies >> Anti Human Cytokine >> IL 2 Antibody
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Description
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Interleukin-2, Mouse Anti-Human
 
Catalogue Number ANT-102
Synonyms T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.
Type Mouse Anti Human Monoclonal.
Introduction IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Clone YNRhIL2.
Immunogen r.Human IL-2.
Ig Subclass Mouse IgG2a.
Purification Method Ion Exchange.
Protein concentration 1mg/ml in PBS (after reconstitution).
Shipping Conditions Antibody is shipped lyophilized at ambient temperature.
Storage Procedures In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Reconstitution Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Stability / Shelf Life Two years lyophilized, one month in solution at 4°C.
Titer By direct ELISA 1:10,000 dilution will yield 0.4 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). 1:50 dilution will block 70-80% of CTLL proliferative response to 50 units of r.human IL-2.
Applications Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry, blocking T cell poroliferation.
Cross reactivity A certain degree of functional cross reactivity with murine IL-2 was reported by some investigators.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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» IL 2r Antibody
» sIL 2R Human
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