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Protein Catalog >> Cytokines >> IL 6 Human
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Interleukin-6 Human Recombinant
 
Catalogue Number CYT-213
Synonyms IFN-b2, B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.
Introduction Il-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of b-cells into ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.
Description Interleukin-6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21000 Dalton.
The IL6 is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Solubility It is recommended to reconstitute the lyophilized Interleukin-6 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro.
Biological Activity The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less than 0.1 ng/ml, corresponding to the specific activity of 1.0 x 10,000,000 Units per mg.
Protein content Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
References 1.Title:Identification of ?-Escin as a Novel Inhibitor of Signal Transducer and Activator of Transcription 3/Janus-Activated Kinase 2 Signaling Pathway that Suppresses Proliferation and Induces Apoptosis in Human Hepatocellular Carcinoma Cells .
Publication: Published online before print April 8, 2010, doi: 10.1124/jpet.110.165498 JPET July 2010 vol. 334 no. 1 285-293
Link:http://jpet.aspetjournals.org/content/334/1/285.full

2.Title:Suppression of Signal Transducer and Activator of Transcription 3 Activation by Butein Inhibits Growth of Human Hepatocellular Carcinoma In Vivo.
Publication:Published OnlineFirst December 3, 2010; doi: 10.1158/1078-0432.CCR-10-1123 Clin Cancer Res March 15, 2011 17; 1425.
Link:http://clincancerres.aacrjournals.org/content/17/6/1425.full

3.Title:Identification of ?-escin as a novel inhibitor of STAT3/JAK2 signaling pathway that suppresses proliferation and induces apoptosis in human hepatocellular carcinoma cells.
Publication:Published online before print April 8, 2010, doi: 10.1124/jpet.110.165498 JPET April 8, 2010 jpet.110.165498
Link:http://jpet.aspetjournals.org/content/early/2010/04/09/jpet.110.165498.short

4.Title:enhanced self -renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene sall4.
Publication:
Link:http://www.biomedcentral.com/content/pdf/1756-8722-4-38.pdf

5.Title:Thymoquinone inhibits proliferation, induces apoptosis and chemosensitizes human multiple myeloma cells through suppression of signal transducer and activator of transcription 3 activation pathway.
Publication:Article first published online: 10 MAY 2010DOI: 10.1111/j.1476-5381.2010.00874.x
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1476-5381.2010.00874.x/full
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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