IL-4 Antibody
Interleukin-4, Rat Anti-Mouse

Synonyms:
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.

Type:
Rat Anti Mouse Monoclonal

Introduction:
IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Clone:
NYRmIL-4

Immunogen:
r.Murine IL-4

Purification Method:
Ion exchange

Protein concentration:
1mg/ml in PBS (after reconstitution)

Shipping Conditions:
Antibody is shipped lyophilized at ambient temperature.

Storage Procedures:
In lyophilized form, for long periods, store at 4^oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20^oC.

Reconstitution:
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Stability / Shelf Life:
Two years lyophilized, one month in solution at 4^oC.

Applications:
Direct ELISA, Western Blot, Immuneprecipitation, Immunohistochemistry, Intracellular staining.

Titer:
By direct ELISA, 1:5,000 dilution will yield 0.4 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

Usage:
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.