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IL 2 Rat

IL 2 Rat

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  • IL 2 Rat

  • Interleukin-2 Rat Recombinant
  • CYT-382
  • Shipped with Ice Packs

Catalogue number

CYT-382

Synonyms

T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.

Introduction

IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

Description

Interleukin-2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 16kDa.

The IL-2 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

The IL-2 solution contains 0.2µm filtered solution in 20mM PB pH3.5 and 30% Glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.

Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Avoid multiple freeze-thaw cycles.

Purity

Greater than 97.0% as determined by SDS-PAGE and HPLC.

Amino acid sequence

APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less than 0.2ng/ml, corresponding to a Specific Activity of 5,000,000IU/mg.

References

Title:Functional Gap Junctions Facilitate Melanoma Antigen Transfer and Cross-Presentation between Human Dendritic Cells.
Publication:The Journal of Immunology June 1, 2007 vol. 178 no. 11 6949-6957
Link:IL 2 Rat prospec publication

Safety Data Sheet

SDS

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Background

Title: Interleukin-2 Rat Recombinant: A Key Cytokine for Immunological Research

 

Abstract:


Interleukin-2 (IL-2) is a critical cytokine that plays a pivotal role in immune responses. This research paper provides a comprehensive analysis of rat recombinant IL-2, focusing on its production, purification, and applications in immunological research. The paper discusses the significance of IL-2 in immune cell proliferation, activation, and regulatory functions. Furthermore, it elucidates the potential therapeutic implications of recombinant IL-2 in immunotherapy and highlights ongoing research in the field. The information presented in this paper aims to enhance the understanding of rat recombinant IL-2 and its utility as a research tool in immunological studies.

 

Introduction:


Interleukin-2 (IL-2) is a cytokine that plays a crucial role in immune regulation, particularly in T cell activation and proliferation. Rat recombinant IL-2, produced through genetic engineering techniques, enables researchers to investigate its biological functions and therapeutic potential.

 

Production and Purification:


Recombinant IL-2 is typically produced using expression systems such as bacteria, yeast, or mammalian cells. The protein is then purified using various chromatographic techniques to obtain a highly pure and biologically active form. Quality control measures ensure the integrity and functional activity of the recombinant protein.

 

Immunological Applications:


Rat recombinant IL-2 has extensive applications in immunological research. It serves as a potent growth factor for T cells, promoting their proliferation and differentiation. IL-2 also plays a critical role in the development and maintenance of regulatory T cells, which are essential for immune tolerance and homeostasis. Recombinant IL-2 is a valuable tool for studying these processes and dissecting the underlying mechanisms.

 

Therapeutic Implications:


The immunomodulatory properties of IL-2 have led to its exploration as a potential therapeutic agent in immunotherapy. Recombinant IL-2 has been investigated for its use in cancer immunotherapy, autoimmune disorders, and infectious diseases. Ongoing research focuses on optimizing IL-2-based therapies, including combination treatments and targeted delivery approaches.

 

Conclusion:


Rat recombinant IL-2 is a fundamental research tool in the field of immunology. Its production, purification, and applications in immune cell proliferation and regulation contribute to our understanding of immune responses and the development of novel therapeutics. Continued research and advancements in IL-2-based immunotherapy hold promise for improving treatment outcomes in various immunological disorders.

References

Bibliography:

 

  1. Rosenberg, S. A., & IL-2: The First Effective Immunotherapy for Human Cancer. Journal of Immunology, 192(12), 5451-5458.
  2. Boyman, O., & Sprent, J. (2012). The role of interleukin-2 during homeostasis and activation of the immune system. Nature Reviews Immunology, 12(3), 180-190.
  3. Malek, T. R. (2008). The biology of interleukin-2. Annual Review of Immunology, 26, 453-479.
  4. Smith, K. A. (1988). Interleukin-2: inception, impact, and implications. Science, 240(4856), 1169-1176.
  5. Nelson, B. H. (2004). IL-2, regulatory T cells, and tolerance. Journal of Immunology, 172(7), 3983-3988.
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