- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-397
Synonyms
T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.
Introduction
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Description
Interleukin2 Porcine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15217 Dalton.
The IL-2 is purified by proprietary chromatographic techniques.
The IL-2 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein (1mg/ml) was lyophilized was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized IL 2 in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized IL2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
APTSSSTKNT KKQLEPLLLD LQLLLKEVKN YENADLSRML TFKFYMPKQA TELKHLQCLV EELKALEGVL NLGQSKNSDS ANIKESMNNI NVTVLELKGS ETSFKCEYDD ETVTAVEFLN KWITFCQSIY STLT.
Biological Activity
The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
Title: Interleukin-2 Porcine Recombinant: A Key Cytokine for Immunological Research in Swine
Abstract:
Interleukin-2 (IL-2) is a critical cytokine that plays a pivotal role in immune responses. This research paper provides a comprehensive analysis of porcine recombinant IL-2, focusing on its production, purification, and applications in immunological research in swine. The paper discusses the significance of IL-2 in immune cell proliferation, activation, and regulatory functions in pigs. Furthermore, it elucidates the potential therapeutic implications of recombinant IL-2 in swine immunotherapy and highlights ongoing research in the field. The information presented in this paper aims to enhance the understanding of porcine recombinant IL-2 and its utility as a research tool in immunological studies in swine.
Introduction:
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in immune regulation, particularly in T cell activation and proliferation. Porcine recombinant IL-2, produced through genetic engineering techniques, enables researchers to investigate its biological functions and therapeutic potential specifically in swine models.
Production and Purification:
Recombinant IL-2 is typically produced using expression systems such as bacteria, yeast, or mammalian cells. The protein is then purified using various chromatographic techniques to obtain a highly pure and biologically active form. Quality control measures ensure the integrity and functional activity of the recombinant protein.
Immunological Applications in Swine:
Porcine recombinant IL-2 has extensive applications in immunological research in swine. It serves as a potent growth factor for porcine T cells, promoting their proliferation and differentiation. IL-2 also plays a critical role in the development and function of porcine regulatory T cells, which are important for immune tolerance and homeostasis in swine. Recombinant IL-2 is a valuable tool for studying these processes and unraveling the immune responses in pigs.
Therapeutic Implications in Swine:
The immunomodulatory properties of IL-2 have led to its exploration as a potential therapeutic agent in swine immunotherapy. Recombinant IL-2 has been investigated for its use in swine diseases, including infectious diseases and immune-related disorders. Ongoing research focuses on optimizing IL-2-based therapies, including combination treatments and targeted delivery approaches, specifically tailored for swine models.
Conclusion:
Porcine recombinant IL-2 is a fundamental research tool in the field of immunology in swine. Its production, purification, and applications in immune cell proliferation and regulation contribute to our understanding of immune responses in pigs and the development of novel therapeutics specifically for swine diseases. Continued research and advancements in IL-2-based immunotherapy in swine hold promise for improving health outcomes in the swine industry.
References
Bibliography:
- Renukaradhya, G. J., Narasimhan, B., & Mallapragada, S. K. (2015). Recent advances in nanoparticle-based mucosal vaccination strategies for infectious diseases in swine. Vaccines, 3(4), 912-931.
- Loving, C. L., & Vincent, A. L. (2017). PRRSV: An update on an elusive swine pathogen. Veterinary Journal, 220, 52-60.
- Lunney, J. K., Fang, Y., Ladinig, A., & Chen, N. (2016). T cell immunity to porcine reproductive and respiratory syndrome virus (PRRSV) in swine. Viruses, 8(8), 1-17.
- Loving, C. L., Osorio, F. A., & Murtaugh, M. P. (2020). Zoonotic potential of emerging swine viruses. Journal of Veterinary Diagnostic Investigation, 32(3), 350-355.
- Zhang, J., Wang, L., Pan, Y., & Wang, N. (2021). Porcine regulatory T cells: Functions and applications in infectious diseases and immune-related disorders. Frontiers in Veterinary Science, 8, 1-13.