- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-370
Synonyms
Interleukin-2, T-cell growth factor (TCGF), Lymphokine, IL-2.
Introduction
Description
The IL-2 is purified by proprietary chromatographic techniques.
Source
Physical Appearance
Formulation
Solubility
Stability
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
Biological Activity
Protein content
1. UV spectroscopy at 280 nm using the absorbency value of 0.482 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.
Safety Data Sheet
Usage
Background
Title: Interleukin-2 Mouse Recombinant: A Key Cytokine in Immunological Research
Abstract:
Interleukin-2 (IL-2) is a critical cytokine that plays a pivotal role in immune responses. This research paper provides a comprehensive analysis of mouse recombinant IL-2, focusing on its production, purification, and applications in immunological research. The paper discusses the significance of IL-2 in immune cell proliferation, activation, and regulatory functions. Furthermore, it elucidates the potential therapeutic implications of recombinant IL-2 in immunotherapy and highlights ongoing research in the field. The information presented in this paper aims to enhance the understanding of mouse recombinant IL-2 and its utility as a research tool in immunological studies.
Introduction:
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in immune regulation, particularly in T cell activation and proliferation. Mouse recombinant IL-2, produced through genetic engineering techniques, enables researchers to investigate its biological functions and therapeutic potential.
Production and Purification:
Recombinant IL-2 is typically produced using expression systems such as bacteria, yeast, or mammalian cells. The protein is then purified using various chromatographic techniques to obtain a highly pure and biologically active form. Quality control measures ensure the integrity and functional activity of the recombinant protein.
Immunological Applications:
Mouse recombinant IL-2 has extensive applications in immunological research. It serves as a potent growth factor for T cells, promoting their proliferation and differentiation. IL-2 also plays a critical role in the development and maintenance of regulatory T cells, which are essential for immune tolerance and homeostasis. Recombinant IL-2 is a valuable tool for studying these processes and dissecting the underlying mechanisms.
Therapeutic Implications:
The immunomodulatory properties of IL-2 have led to its exploration as a potential therapeutic agent in immunotherapy. Recombinant IL-2 has been investigated for its use in cancer immunotherapy, autoimmune disorders, and infectious diseases. Ongoing research focuses on optimizing IL-2-based therapies, including combination treatments and targeted delivery approaches.
Conclusion:
Mouse recombinant IL-2 is a fundamental research tool in the field of immunology. Its production, purification, and applications in immune cell proliferation and regulation contribute to our understanding of immune responses and the development of novel therapeutics. Continued research and advancements in IL-2-based immunotherapy hold promise for improving treatment outcomes in various immunological disorders.
References
Bibliography:
- Rosenberg, S. A., & IL-2: The First Effective Immunotherapy for Human Cancer. Journal of Immunology, 192(12), 5451-5458.
- Boyman, O., & Sprent, J. (2012). The role of interleukin-2 during homeostasis and activation of the immune system. Nature Reviews Immunology, 12(3), 180-190.
- Malek, T. R. (2008). The biology of interleukin-2. Annual Review of Immunology, 26, 453-479.
- Smith, K. A. (1988). Interleukin-2: inception, impact, and implications. Science, 240(4856), 1169-1176.
- Nelson, B. H. (2004). IL-2, regulatory T cells, and tolerance. Journal of Immunology, 172(7), 3983-3988.