- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-797
Synonyms
Interleukin-2, T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.
Introduction
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Description
Interleukin-2 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 14 kDa. The IL-2 is purified by proprietary chromatographic techniques.
Source
Pichia pastoris.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a 0.2µm filtered solution in 20mM sodium phosphate buffer pH 7.0 in absence of any carrier protein.
Solubility
It is recommended to reconstitute the lyophilized Interleukin-2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Interleukin-2 should be stored at 4C between 2-7 days and for future use below -18C.
Please prevent freeze-thaw cycles.
Please prevent freeze-thaw cycles.
Purity
Greater than 98% as determined by SDS-PAGE.
Amino acid sequence
A P T S S S T K K T Q L Q L E H L L L D L Q M I L N G I N N Y K N P K L T R M L T F K F Y M P K K A T E L K H L Q C L E E E L K P L E E V L N L A Q S K N F H L R P R D L I S N I N V I V L E L K G S E T T F M C E Y A D E T A T I V E F L N R W I T F C Q S I I S T L T.
Biological Activity
The biological activity is determined by the dose dependent proliferation of mouse CTLL–2 cells. Optimal concentration for individual application should be determined by a dose response assay. ED50 range = 0.08–0.5ng/ml
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
Title: Interleukin-2 Human Recombinant, Yeast: A Promising Biopharmaceutical for Immunotherapy
Abstract:
Interleukin-2 (IL-2) is a potent cytokine that regulates immune responses and has shown promising therapeutic efficacy in various diseases. The development of IL-2 human recombinant using yeast expression systems has provided a valuable biopharmaceutical tool for immunotherapy. This research paper explores the production process, characteristics, and therapeutic applications of IL-2 human recombinant derived from yeast, highlighting its potential as an immunotherapeutic agent.
Introduction:
IL-2 is a critical cytokine involved in the regulation of immune responses, including the activation and proliferation of T cells and natural killer (NK) cells. The use of IL-2 as a therapeutic agent has been limited by challenges associated with production and stability. However, the development of IL-2 human recombinant using yeast expression systems has overcome these limitations, making it an attractive biopharmaceutical for immunotherapy.
Production Process and Characteristics:
IL-2 human recombinant derived from yeast is produced through recombinant DNA technology, utilizing yeast cells as expression hosts. Yeast expression systems offer advantages such as high expression levels, cost-effectiveness, and post-translational modifications that resemble those in human cells. The resulting IL-2 human recombinant possesses similar biological activity to native IL-2, with the ability to bind to IL-2 receptors and stimulate immune cell proliferation and activation.
Therapeutic Applications:
IL-2 human recombinant derived from yeast has shown promise in the treatment of several diseases. It has been investigated as an immunotherapeutic agent for cancer, particularly in combination with other treatment modalities such as immune checkpoint inhibitors. IL-2 human recombinant has also demonstrated potential in the treatment of autoimmune disorders, including rheumatoid arthritis and psoriasis. Furthermore, it has been explored in the context of infectious diseases and as an adjuvant in vaccines.
Advantages and Challenges:
The use of yeast expression systems for producing IL-2 human recombinant offers several advantages, including scalability, cost-effectiveness, and the ability to generate post-translational modifications that enhance biological activity. However, challenges remain, such as optimizing expression yields, purification processes, and ensuring product consistency and stability. Further research is needed to address these challenges and improve the overall feasibility and clinical efficacy of IL-2 human recombinant derived from yeast.
Conclusion:
IL-2 human recombinant derived from yeast represents a promising biopharmaceutical tool for immunotherapy. Its production using yeast expression systems offers advantages in terms of scalability, cost-effectiveness, and biological activity. The therapeutic applications of IL-2 human recombinant extend to various diseases, including cancer and autoimmune disorders. Continued research and development efforts are crucial to optimizing production processes, addressing challenges, and unlocking the full potential of IL-2 human recombinant as an immunotherapeutic agent.
References
Bibliography:
- Boyman O, Sprent J. The role of interleukin-2 during homeostasis and activation of the immune system. Nat Rev Immunol. 2012;12(3):180-190.
- Atkins MB, Lotze MT, Dutcher JP, et al. High-dose recombinant interleukin 2 therapy for patients with metastatic melanoma: analysis of 270 patients treated between 1985 and 1993. J Clin Oncol. 1999;17(7):2105-2116.
- Waldmann TA. The biology of interleukin-2 and interleukin-15: implications for cancer therapy and vaccine design. Nat Rev Immunol. 2006;6(8):595-601.
- Charych DH, Hoch U, Langowski JL, et al. NKTR-214, an engineered cytokine with biased IL2 receptor binding, increased tumor exposure, and marked efficacy in mouse tumor models. Clin Cancer Res. 2016;22(3):680-690.
- Rosenberg SA, Yang JC, White DE, et al. Durability of complete responses in patients with metastatic cancer treated with high-dose interleukin-2: identification of the antigens mediating response. Ann Surg. 1998;228(3):307-319.