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Protein Catalog >> Monoclonal Antibodies >> Anti Human Cytokine >> IL 1 Beta Antibody, FITC
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Description
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Interleukin-1b, Mouse Anti-Human FITC
 
Catalogue Number ANT-250
Synonyms Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Type Mouse Anti Human Monoclonal.
Introduction Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Clone NYR-hIL1?.
Immunogen r.Human IL-1b.
Ig Subclass Mouse IgG2b.
Purification Method Ion Exchange.
Protein concentration 1 mg/ml in PBS (after reconstitution).
Shipping Conditions Antibody is shipped lyophilized at ambient temperature.
Storage Procedures In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Reconstitution Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C.
Patent Rights The sale and/or commercial use of Recombinant Human IL1B is prohibited in the United States of America (U.S.A) and Japan.
Applications Western Blot, immunohistochemistry intra-cellular staining and will also work in Direct ELISA and Immuneprecipitation.
Titer For intra-cellular staining, 10µl per 1,000,000 cells. By direct ELISA, 1:10,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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