IL 12 Human | ProSpec

Description

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Quantity

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Interleukin-12 Human Recombinant

	2µg($50)
	10µg($130)
	0.1mg($990)

Catalogue Number

CYT-362

Synonyms

NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.

Introduction

IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. IL-12 is an initiator of cell-mediated immunity.

Description

Interleukin-12 Human Recombinant produced in CHO is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
The IL-12 is purified by proprietary chromatographic techniques.

Source

CHO (Chinese Hamster Ovarian Cells).

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

IL-12 was lyophilized from a 0.2?m filtered solution (0.5mg/ml) in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized IL-12 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized IL-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-12 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 98% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids of IL-12 was determined and found to be Arg-Asn-Leu-Pro-Val at the p35 subunit and Ile-Trp-Glu-Leu-Lys at the p40 subunit.

Biological Activity

IL-12 activity was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. The ED50 for this effect is 0.3-0.5 ng/ml corresponding to a specific activity of 2-3.3MU/mg.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.