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Protein Catalog >> Monoclonal Antibodies >> Anti Mouse Cytokine >> IL 10 Antibody
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Description
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Interleukin-10, Rat Anti-Mouse
 
Catalogue Number ANT-113
Synonyms B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Type Rat Anti Mouse Monoclonal.
Introduction IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Clone NYRmIL-10.
Immunogen r.Murine IL-10.
Ig Subclass Rat IgM.
Purification Method Boric Acid precipitation.
Protein concentration 1mg/ml in PBS (after reconstitution).
Shipping Conditions Antibody is shipped lyophilized at ambient temperature.
Storage Procedures In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.
Reconstitution Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Applications Direct ELISA, Western Blot, Immuneprecipitation Immunohistochemistry, Intracellular staining.
Titer By direct ELISA, 1:5,000 dilution will yield 0.1 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).
Usage Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
» IL 10 Antibody
» IL 10 Human
» IL 10 Human, HEK
» IL 10 Human, His
» IL 10 Mouse
» IL 10 Rat
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