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Products catalog >> Monoclonal Antibodies >> Anti Mouse Cytokine >> IL-10 Antibody

IL-10 Antibody
Interleukin-10, Rat Anti-Mouse

Catalog No. ANT-113
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Synonyms:
B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

Type:
Rat Anti Mouse Monoclonal

Introduction:
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Clone:
NYRmIL-10

Immunogen:
r.Murine IL-10

Ig Subclass:
Rat IgM

Purification Method:
Boric Acid precipitation

Protein concentration:
1mg/ml in PBS (after reconstitution)

Shipping Conditions:
Antibody is shipped lyophilized at ambient temperature.

Storage Procedures:
In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.

Reconstitution:
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Stability / Shelf Life:
Two years lyophilized, one month in solution at 4oC.

Applications:
Direct ELISA, Western Blot, Immuneprecipitation Immunohistochemistry, Intracellular staining.

Titer:
By direct ELISA, 1:5,000 dilution will yield 0.1 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

Usage:
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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» IL-10 Rat
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