Interleukin 10 Human

Description

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Quantity

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Interleukin-10 Human Recombinant

	2µg($50)
	10µg($130)
	1mg($4680)

Catalogue Number

CYT-500

Synonyms

B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

Introduction

IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Description

Interleukin-10 Human Recombinant produced in E.coli is a non covalent dimer of 2 polypeptide chains containing 161 amino acids each and having a molecular mass of 18606.29 Dalton.
The IL-10 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution containing no additives.

Solubility

It is recommended to reconstitute the lyophilized Interleukin-10 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Interleukin-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of mouse MC-9 cells was found to be < 0.5ng/ml, corresponding to a Specific Activity of 2MIU/mg.

References

Title:Activated signal transducer and activator of transcription-3 (STAT3) is a poor regulator of tumour necrosis factor- [alpha]

production by human monocytes.
Publication:Article first published online: 9 JAN 2007 DOI:10.1111/j.1365-2249.2006.03291.x
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2006.03291.x/full

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.