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Catalogue Number
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CYT-019
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Synonyms
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Interferon alpha 2b, IFNA, INFA2, MGC125764, MGC125765.
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Introduction
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IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.
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Description
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Interferon-a 2b Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19269 Dalton. The Pegylated IFN-a 2b is produced by attaching 40kDa NHS-mPEG to the primary amino acid of IFN alpha-2b.
Interferon-a 2b is purified by proprietary chromatographic techniques.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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IFN-a 2b was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 150mM NaCl, 0.01% EDTA and 0.01% Tween 80 buffer.
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Solubility
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It is recommended to reconstitute the lyophilized Interferon alpha 2b in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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Stability
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Lyophilized Interferon although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution the Pegylated IFN alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
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Amino acid sequence
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The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asp-Leu-Pro.
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Purity
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Greater than 98.0% as determined by:
a) Analysis by RP-HPLC.
b) Analysis by SDS-PAGE.
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Biological Activity
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The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be 1,000,000 IU/mg.
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Usage
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ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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