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Horseradish Peroxidase
Catalogue Number ENZ-321
Synonyms Horseradish Peroxidase, HRP, EC
Introduction The enzyme horseradish peroxidase, found in horseradish, is used extensively in molecular biologyand in antibody amplification and detection, among other things. For example, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase (HRP) has become a major tool. In its brief history, this method has probably been used by more neurobiologists than have used the Golgi stainsince its discovery in 1870." Horseradish peroxidase is also highly used in techniques such as Western blottingand ELISAs.
HRP is widely used as an enzymatic label in immunoassays. Usually, the enzyme is coupled to antibodies, lectins or haptens. Coupling to antibodies etc. may be performed through the carbohydrate side chains of the HRP.
Description HRP consists of the basic isoenzyme having a molecular weight of 44 kDa.
The Horseradish Peroxidase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Source Root extracts of horseradish.
Physical Appearance Sterile Filtered red-brown lyophilized powder.
Solubility It is recommended to reconstitute the lyophilized HRP in sterile 18MΩ-cm H2O not less than 100 µg/ml or more than 10 mg/ml solutions.
Stability Lyophilized HRP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HRP should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Activity Greater than 268 U/mg (25°C, guaiacol as the hydrogen donor, pH-7 and H2O2 as substrates).
Purity (A403/A275) = RZ: 3.5.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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