HDV
Hepatitis D Virus Recombinant

Introduction:
The HDV genome exists as a negative sense, single-stranded, closed circular RNA. Because of a nucleotide sequence that is 70% self-complementary, the HDV genome forms a partially double stranded RNA structure that is described as rod-like. With a genome of approximately 1700 nucleotides, It has been proposed that HDV may have originated from a class of plant viruses called viroids. Evidence in support of this hypothesis stems from the fact that both HDV and viroids exist as single-stranded, closed circular RNAs that have rod-like structures. Likewise, both HDV and viroids contain RNA sequences that can assume catalytically active structures called ribozymes.

Description:
The E.Coli derived recombinant protein contains the HDV immunodominant regions, amino acids 1-108aa, 151-209aa.

Method:
Purified by proprietary chromatographic technique.

Purity:
Protein is >90% pure as determined by 10% PAGE (Coomassie staining).

Formulation:
10mM carbonate buffer pH 10.0, NaCl 100mM and 50% glycerol.

Storage:
Protein is shipped at ambient temperature.
Upon arrival, Store at -20°C.

Stability:
Five years frozen. One month in solution at room temperature.

Specificity:
Immunoreactive with sera HDV-infected individuals.

Applications:
Antigen in ELISA and Western blots, excellent antigen for detection of HDV with minimal specificity problems.

USAGE:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals