HTLV-1 Envelope

Description

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Quantity

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HTLV-1 Envelope Recombinant

	100µg($150)
	500µg($600)
	1000µg($1000)

Catalogue Number

HIV-107

Introduction

Human T-lymphotropic virus (HTLV) is a human, single-stranded RNA retrovirus that causes T-cell leukemia and T-cell lymphoma. The virus activates a subset of T-helper cellscalled Th1cells. The result is a proliferation of Th1 cells and overproduction of Th1 related cytokines (mainly IFN-gamma and TNF-alpha). Feedback mechanisms of these cytokines cause a suppression of the Th2 lymphocytes and a reduction of Th2 cytokine production (mainly IL-4, IL-5, IL-10 and IL-13). The end result is a reduction in the ability of the infected host to mount an adequate immune response to invading organisms that require a predominantly Th2 dependant response (these include parasitic infections and production of mucosal and humoral antibodies).

Description

The E.Coli derived recombinant protein contains the C- terminus of gp46 and most of p21E of HTLV-1. This non-fusion, E. coli-derived protein, starts from HIV-1 env. Amino acid 165, and is ending in amino acid 440, Mw 27 kDa.

Purification Method

HTLV-1 Envelope was purified by proprietary chromatographic technique.

Purity

HTLV-1 Envelope protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation

10mM Na-PO4 pH 6.0, 0.1% SDS and 1mM DTT.

Solubility

It is recommended to reconstitute the lyophilized HTLV-1 Envelope in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized HTLV-1 Envelope although stable at room temperature for 3 weeks, should be stored desiccated below - 18°C. Upon reconstitution HTLV-1 Envelope should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Specificity

Immunoreactive with all sera of HTLV-I and HTLV-II infected individuals with antibody response to HTLV envelope.

Applications

HTLV-1 Envelope can be used as an antigen in ELISA and Western Blots. Excellent reagent for correct detection of HTLV infections, with minimal specificity problems.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.