|
|
|
|
|
|
|
|
|
|
|
|
|
Catalogue Number
|
HIV-002
|
|
Introduction
|
HIV-2 protease is very significant in the life cycle of the HIV virus. It is expressed in the infected cells as a part of Gag-Pol polyprotein from which it is auto-catalytycaly released after formation of an immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins resulting in the maturation of the virus. In HIV-infected patients the enzyme is subjected to intensive mutagenesis and mutants resistant to applied medicines are produced as a result of the selection pressure.
|
|
Description
|
HIV-2 protease is an active homodimer having a molecular mass of 10.7kDa.
|
|
Source
|
Escherichia Coli.
|
|
Physical Appearance
|
Sterile filtered colorless clear solution.
|
|
Formulation
|
The HIV-2 Protease solution (0.25mg/1ml) is formulated in 20mM Tris pH7.0, 20mM MES, 200mM NaCl, 1mM EDT, 0.5mM DTT, 0.05% PEG 8000and 10% glycerol.
|
|
storage buffer
|
20mM HEPES, pH7.0 and 10% Glycerol.
|
|
Stability
|
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
|
|
Kinetic parameters
|
Km=740µM, Kcat = 30s-1, Kcat/Km=4.1mM-1s-1 with peptide substrate ATLNFPISPW.
|
|
Usage
|
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
|
|
Related Products
|
|
|
|
|
|
|
| Contact Us |
 |
Toll Free Phone: 1-(866)-245-0885
Toll Free Fax: 1-(866)-796-4944
Postal Address: PO Box 6591,
East Brunswick, 08816 NJ |
 |
Toll Free Phone: 00-800-800-66666
Toll Free Fax: 00-800-800-67890 |
 |
International Phone: +972-8-9471175
International Fax: +972-8-9460534
Postal Address: PO Box 4157,
Ness-Ziona, 74140 Israel
|
|
 |
Website : www.prospecbio.com
Email: info@prospecbio.com |
|
|
|
|
|
|
|