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Catalogue Number
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HIV-001
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Introduction
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HIV-1 protease is very significant in the life cycle of the HIV virus. It is expressed in the infected cells as a part of Gag-Pol polyprotein from which it is auto-catalytycaly released after formation of an immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins resulting in the maturation of the virus. In HIV-infected patients the enzyme is subjected to intensive mutagenesis and mutants resistant to applied medicines are produced as a result of the selection pressure.
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Description
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HIV-1 protease is an active homodimer having a molecular mass of 21.6kDa (each monomer of 99 amino acids is 10.8kDa).
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile filtered colorless clear solution.
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Formulation
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The HIV-1 Protease solution (0.25mg/1ml) is formulated in 20mM acetate, 200mM NaCl, 1mM EDTA, 0.5mM DTT, pH5.0 and 10% glycerol.
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Storage Buffer
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20mM HEPES, pH7.0 and 10% Glycerol.
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Stability
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Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
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Kinetic parameters
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Km=15.1µM, Kcat = 30s-1, Kcat/Km= 1981 mM-1s-1 with peptide substrate KARVF(NO2)VRKA (F(NO2) ... p-nitrophenylalanine).
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Usage
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ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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| Contact Us |
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Toll Free Phone: 1-(866)-245-0885
Toll Free Fax: 1-(866)-796-4944
Postal Address: PO Box 6591,
East Brunswick, 08816 NJ |
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Toll Free Phone: 00-800-800-66666
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International Phone: +972-8-9471175
International Fax: +972-8-9460534
Postal Address: PO Box 4157,
Ness-Ziona, 74140 Israel
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Website : www.prospecbio.com
Email: info@prospecbio.com |
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