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Hepatitis E virus (HEV), the major etiologic agent of enterically transmitted non-A, non-B hepatitis worldwide, is a spherical, non-enveloped, single stranded RNA virus that is approximately 32 to 34 nm in diameter. HEV belongs to a genus of HEV-like viruses (unassigned genus). HEV has a single-stranded polyadenylated RNA genome of approximately 8 kb. Based on its physicochemical properties it is presumed to be a calici-like virus. The e.coli derived HEV protein is fused with beta-galactosidase at the N-Terminus and contains the HEV immunodominant regions from ORF2 403-461 a.a.
HEV ORF2 was purified by proprietary chromatographic technique.
HEV ORF2 Protein is >95% pure as determined by 10% PAGE (coomassie staining).
20mM Tris-HCl pH-8, 8M urea and 10mM B-ME.
HEV ORF2 protein is shipped at ambient temperature. Upon arrival, Store at -20°C.
Five years frozen. One month in solution at room temperature.
Immunoreactive with sera HEV-infected individuals.
HEV ORF2 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HEV with minimal specificity problems.
ProSpec's products are furnished for LABORATORY RESEARCHUSEONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
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